Structure of PDB 3wiv Chain A

Receptor sequence

>3wivA (length=391) Species: 69014 (Thermococcus kodakarensis KOD1)

NTIRVIVSVDKAKFNPHEVLGIGGHIVYQFKLIPAVVVDVPANAVGKLKK
MPGVEKVEFDHQAVLLGKPSWSTQPAQTIPWGIERVKAPSVWSITDGSVS
VIQVAVLDTGVDYDHPDLAANIAWCVSTLRGKVSTKLRDCADQNGHGTHV
IGTIAALNNDIGVVGVAPGVQIYSVRVLDARGSGSYSDIAIGIEQAILGP
DGVADKDGDGIIAGDPDDDAAEVISMSLGGPADDSYLYDMIIQAYNAGIV
IVAASGNEGAPSPSYPAAYPEVIAVGAIDSNDNIASFSNRQPEVSAPGVD
ILSTYPDDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKILPVGTFDAI
SKNTVRGILHITADDLGPTGWDADYGYGVVRAALAVQAALG

3D structure

• PDB: 3wiv Requirement of the insertion sequence for activation of Tk-subtilisin
• Chain: A
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D115 H153 N264 A324
• Catalytic site (residue number reindexed from 1): D108 H146 N257 A317
• Enzyme Commision number: 3.4.21.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	Q84 D124 L164 N166 I168 V170	Q77 D117 L157 N159 I161 V163
BS02	CA	A	L205 D208 V210 D226	L198 D201 V203 D219
BS03	CA	A	D212 D214 D216 I218 D222 D225	D205 D207 D209 I211 D215 D218
BS04	CA	A	D214 D216 D222 D224	D207 D209 D215 D217
BS05	CA	A	V108 Q110 A227 E229	V101 Q103 A220 E222
BS06	CA	A	D372 L373 P375 G377 D379	D365 L366 P368 G370 D372

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3wiv, PDBe:3wiv, PDBj:3wiv
• PDBsum: 3wiv
• UniProt: P58502|TKSU_THEKO Tk-subtilisin (Gene Name=TK1675)