Structure of PDB 3wiu Chain A

Receptor sequence

>3wiuA (length=395) Species: 69014 (Thermococcus kodakarensis KOD1)

NTIRVIVSVDKAKFNPHEVLGIGGHIVYQFKLIPAVVVDVPANAVGKLKK
MPGVEKVEFDHQAVLLGKPSWLGGGSTQPAQTIPWGIERVKAPSVWSITD
GSVSVIQVAVLDTGVDYDHPDLAANIAWCVSTLRGKVSTKLRDCADQNGH
GTHVIGTIAALNNDIGVVGVAPGVQIYSVRVLDARGSGSYSDIAIGIEQA
ILGPDGVADKDGDGIIAGDPDDDAAEVISMSLGGPADDSYLYDMIIQAYN
AGIVIVAASGNEGAPSPSYPAAYPEVIAVGAIDSNDNIASFSNRQPEVSA
PGVDILSTYPDDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKIAPVGT
FDDISKNTVRGILHITADDLGPTGWDADYGYGVVRAALAVQAALG

3D structure

• PDB: 3wiu Requirement of the insertion sequence for activation of Tk-subtilisin
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D115 H153 N264 A324
• Catalytic site (residue number reindexed from 1): D112 H150 N261 A321
• Enzyme Commision number: 3.4.21.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	Q84 D124 L164 N166 I168 V170	Q81 D121 L161 N163 I165 V167
BS02	CA	A	L205 D208 V210 D226	L202 D205 V207 D223
BS03	CA	A	D212 D214 D216 I218 D222 D225	D209 D211 D213 I215 D219 D222
BS04	CA	A	D214 D216 D222 D224	D211 D213 D219 D221
BS05	CA	A	V108 Q110 A227 E229	V105 Q107 A224 E226
BS06	CA	A	D372 L373 P375 G377 D379	D369 L370 P372 G374 D376

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3wiu, PDBe:3wiu, PDBj:3wiu
• PDBsum: 3wiu
• UniProt: P58502|TKSU_THEKO Tk-subtilisin (Gene Name=TK1675)