Structure of PDB 3wie Chain A

Receptor sequence

>3wieA (length=360) Species: 273116 (Thermoplasma volcanium GSS1)

STINAIVTDAPKGGVKYTKIDMPEPEKYEAKLKPVYIGICGTDRGEVAGA
LSFTYNPEGENFLVLGHEALLQVLDVSDNNYIKRGDFVVPLVRRPGKCVN
CRIGRQDNCSIGDPDKHEAGITGLHGFMRDVIYDDIQNLVKVNDPDLGKI
AVLTEPLKNVMKAFEVFDVVSKRSIFQNDDSTFIGKKMVVIGSGSEAFLY
SFVGKDRGFDVTMVNRHDETENKMKMMDDFGVGFSNYLKDMPDKIDLLVD
TSGDPSTIFKFVKKVNNNGVVILFGFNGKAPGYPVNGEDIDYIVERNITI
AGSVDAAKIHYVQALDSLSNWYHRHPQTIKDIITYEAKPEETNIFFQKPK
GEIKTVIKWP

3D structure

• PDB: 3wie Structural insight into glucose dehydrogenase from the thermoacidophilic archaeon Thermoplasma volcanium.
• Chain: A
• Resolution: 2.33 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.47: glucose 1-dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C41 E69	C40 E68
BS02	ZN	A	C99 C102 C110 D116	C98 C101 C109 D115
BS03	DN4	A	N160 G193 S194 G195 S196 E197 N216 R217 H218 Y238 T252 S253 T258 F275 G276 F277 V305	N159 G192 S193 G194 S195 E196 N215 R216 H217 Y237 T251 S252 T257 F274 G275 F276 V304
BS04	BGC	A	T43 E156 D306	T42 E155 D305

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005536 D-glucose binding
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0047934 glucose 1-dehydrogenase (NAD+) activity
• GO:0047935 glucose 1-dehydrogenase (NADP+) activity
• GO:0047936 glucose 1-dehydrogenase [NAD(P)+] activity
• GO:0070401 NADP+ binding
• GO:0070403 NAD+ binding

Biological Process

• GO:0019595 non-phosphorylated glucose catabolic process

External links

• PDB: RCSB:3wie, PDBe:3wie, PDBj:3wie
• PDBsum: 3wie
• PubMed: 24816096
• UniProt: Q979W2