Structure of PDB 3wgu Chain A

Receptor sequence

>3wguA (length=994) Species: 9823 (Sus scrofa)

RDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAEILARDGPNA
LTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIQAATEEEPQNDN
LYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMS
INAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSP
DFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEG
GQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIV
ANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTG
TLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCN
RAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYTKIVE
IPFNSTNKYQLSIHKNPNTAEPRHLLVMKGAPERILDRCSSILIHGKEQP
LDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFP
LDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAK
GVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLD
DILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKAD
IGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYT
LTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAES
DIMKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFL
PIHLLGLRVNWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVV
QWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALR
MYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

3D structure

• PDB: 3wgu Crystal structure of a Na1-bound Na1,K1-ATPase preceding the E1P state
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D369 D710 D714
• Catalytic site (residue number reindexed from 1): D347 D688 D692
• Enzyme Commision number: 7.2.2.13: Na(+)/K(+)-exchanging ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ALF	A	D369 K370 T371 T610 K691 N713	D347 K348 T349 T588 K669 N691
BS02	MG	A	D369 D710 G711	D347 D688 G689
BS03	ADP	A	T371 D443 E446 F475 S477 K480 Q482 K501 R544 L546 G611 D612 R685	T349 D421 E424 F453 S455 K458 Q460 K479 R522 L524 G589 D590 R663
BS04	CLR	A	F860 T979 P986	F838 T957 P964
BS05	CLR	A	Y963 W981 F985	Y941 W959 F963
BS06	CLR	A	S844 G848	S822 G826

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005215 transporter activity
• GO:0005391 P-type sodium:potassium-exchanging transporter activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008556 P-type potassium transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0030955 potassium ion binding
• GO:0031402 sodium ion binding
• GO:0046872 metal ion binding
• GO:0051117 ATPase binding

Biological Process

• GO:0002028 regulation of sodium ion transport
• GO:0006813 potassium ion transport
• GO:0006814 sodium ion transport
• GO:0006883 intracellular sodium ion homeostasis
• GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
• GO:0030007 intracellular potassium ion homeostasis
• GO:0036376 sodium ion export across plasma membrane
• GO:0055085 transmembrane transport
• GO:0086009 membrane repolarization
• GO:1902600 proton transmembrane transport
• GO:1990573 potassium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0005890 sodium:potassium-exchanging ATPase complex
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane
• GO:0030424 axon
• GO:0042383 sarcolemma
• GO:0042470 melanosome
• GO:0042995 cell projection

External links

• PDB: RCSB:3wgu, PDBe:3wgu, PDBj:3wgu
• PDBsum: 3wgu
• UniProt: P05024|AT1A1_PIG Sodium/potassium-transporting ATPase subunit alpha-1 (Gene Name=ATP1A1)