Structure of PDB 3w8d Chain A

Receptor sequence

>3w8dA (length=260) Species: 511 (Alcaligenes faecalis)

MLKGKKAVVTGSTSGIGLAMATELAKAGADVVINGFGQPEDIERERSTLE
SKFGVKAYYLNADLSDAQATRDFIAKAAEALGGLDILVNNAGIQHTAPIE
EFPVDKWNAIIALNLSAVFHGTAAALPIMQKQGWGRIINIASAHGLVASV
NKSAYVAAKHGVVGLTKVTALENAGKGITCNAICPGWVRTPLVEKQIEAI
SQQKGIDIEAAARELLAEKQPSLQFVTPEQLGGAAVFLSSAAADQMTGTT
LSLDGGWTAR

3D structure

• PDB: 3w8d X-ray diffraction of D-3-hydroxybutyrate dehydrogenase from Alcaligenes faecalis complexed with NAD+ and methylmalonate
• Chain: A
• Resolution: 1.37 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G15 N114 S142 Y155 K159 I200
• Catalytic site (residue number reindexed from 1): G15 N114 S142 Y155 K159 I200
• Enzyme Commision number: 1.1.1.30: 3-hydroxybutyrate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	G11 T13 S14 G15 I16 F36 A62 D63 L64 N90 A91 G92 L113 S142 Y155 K159 P185 G186 W187 V188 T190 L192 V193	G11 T13 S14 G15 I16 F36 A62 D63 L64 N90 A91 G92 L113 S142 Y155 K159 P185 G186 W187 V188 T190 L192 V193
BS02	DXX	A	Q94 S142 H144 K152 Y155 W187 Q196 W257	Q94 S142 H144 K152 Y155 W187 Q196 W257

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003858 3-hydroxybutyrate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

External links

• PDB: RCSB:3w8d, PDBe:3w8d, PDBj:3w8d
• PDBsum: 3w8d
• UniProt: D0VWQ0