Structure of PDB 3w5n Chain A

Receptor sequence

>3w5nA (length=1029) Species: 227882 (Streptomyces avermitilis MA-4680 = NBRC 14893)

ALRVTSPSVEYVQRPLGLDAAHPRLSWPMASAAPGRRQSAYQVRVASSAA
GLSHPDVWDSGKVVSDDSVLVPYAGPPLKPRTRYFWSVRVWDADGGASEW
SAPSWWETGLMGASQWSAKWISAPAPLTEAPSLEGSSWIWFPEGEPANSA
PAATRWFRRTVDLPDDITGATLAISADNVYAVSVDGAEVARTDLEADNEG
WRRPAVIDVLDHVHSGNNTLAVSASNASVGPAGWICVLVLTTASGEKKIF
SDASWKSTDHEPADGWREPDFDDSGWPAAKVAAAWGAGPWGRVAPVASAA
NQLRHEFRLPHKKVSRARLYATALGLYEAHLNGRRVGRDQLAPGWTDYRK
RVQYQTYDVTSSVRPGANALAAYVAPGWYAGNVGMFGPHQYGERPALLAQ
LEVEYADGTSERITSGPDWRAASGPIVSADLLSGETYDARKETAGWTSPG
FDDRAWLAVRGADNDVPEQIVAQVDGPVRIAKELPARKVTEPKPGVFVLD
LGQNMVGSVRLRVSGDAGTTVRLRHAEVLNPDGTIYTANLRSAAATDTYT
LKGQGEETYEPRFTFHGFRYVEVTGFPGKPSTTSVTGRVMHTSAPFTFEF
ETNVPMLNKLHSNITWGQRGNFLSVPTDTPARDERLGWTGDINVFAPTAA
YTMESARFLTKWLVDLRDAQTSDGAFTDVAPAVGNLGNGVAGWGDAGVTV
PWALYQAYGDRQVLADALPSVHAWLRYLEKHSDGLLRPADGYGDWLNVSD
ETPKDVIATAYFAHSADLAARMATELGKDAAPYTDLFTRIRKAFQTAYVA
SDGKVKGDTQSAYVLTLSMNLVPDALRKAAADRLVALIEAKDWHLSTGFL
GTPRLLPVLTDTGHTDVAYRLLHQRTFPSWGYPIDKGSTTMWERWDSIQP
DGGFQTPEMNSFNHYAYGSVGEWMYANIAGIAPGRAGYRQVVIRPRPGGE
VTSARATFASLHGPVSTRWQQRSGGFVLTCSVPPNTTAEVWIPADHPDRV
QHTHGTFVRAEDGCAVFEVGSGSHRFTVK

3D structure

• PDB: 3w5n The structure of a Streptomyces avermitilis alpha-L-rhamnosidase reveals a novel carbohydrate-binding module CBM67 within the six-domain arrangement.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.40: alpha-L-rhamnosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D179 N180 N228 P233	D177 N178 N226 P231
BS02	RAM	A	D630 E636 W640 D643 W695 Y744 W747 H916	D628 E634 W638 D641 W693 Y742 W745 H914
BS03	RAM	A	D179 N180 W203 P233	D177 N178 W201 P231
BS04	RAM	A	K484 F598 R621	K482 F596 R619
BS05	RAM	A	T162 P271	T160 P269
BS06	RAM	A	R351 Q392 Y393 V685 G686 N687	R349 Q390 Y391 V683 G684 N685

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity
• GO:0030596 alpha-L-rhamnosidase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:3w5n, PDBe:3w5n, PDBj:3w5n
• PDBsum: 3w5n
• PubMed: 23486481
• UniProt: Q82PP4|RHA78_STRAW Alpha-L-rhamnosidase (Gene Name=SAVERM_828)