Structure of PDB 3w0s Chain A

Receptor sequence
>3w0sA (length=298) Species: 562 (Escherichia coli) [Search protein sequence]
LTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCAD
GFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIA
DPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVL
TDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPE
LAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGT
3D structure
PDB3w0s Crystal structures of the ternary complex of APH(4)-Ia/Hph with hygromycin B and an ATP analog using a thermostable mutant.
ChainA
Resolution1.77 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.1.163: hygromycin B 4-O-kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HY0 A Q101 D198 S201 N202 E219 N231 W235 W238 L239 Q273 D285 Q96 D193 S196 N197 E214 N226 W230 W233 L234 Q268 D280
BS02 ANP A S29 A36 V47 P75 R94 A95 N202 S24 A31 V42 P70 R89 A90 N197
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0016301 kinase activity
Biological Process
GO:0016310 phosphorylation
GO:0046677 response to antibiotic

View graph for
Molecular Function
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Biological Process
External links
PDB RCSB:3w0s, PDBe:3w0s, PDBj:3w0s
PDBsum3w0s
PubMed23747390
UniProtP00557|KHYB_ECOLX Hygromycin-B 4-O-kinase (Gene Name=hph)

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