Structure of PDB 3w0q Chain A

Receptor sequence
>3w0qA (length=296) Species: 562 (Escherichia coli) [Search protein sequence]
ATSVEKFLIEKFGSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGF
YKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETE
LPAVLQPVAEVMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADP
HVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNAVLTD
NGRITAVIDWSEAMFGDPLYEVANIFFWRPWLACMEQQARYFERRHPELA
GSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGT
3D structure
PDB3w0q Crystal structures of the ternary complex of APH(4)-Ia/Hph with hygromycin B and an ATP analog using a thermostable mutant.
ChainA
Resolution1.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.1.163: hygromycin B 4-O-kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ANP A S29 V47 P75 R94 A95 L205 I215 S22 V40 P68 R87 A88 L198 I208
BS02 HY0 A Q101 D198 S201 N202 N231 W235 W238 L239 D285 Q94 D191 S194 N195 N224 W228 W231 L232 D278
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0016301 kinase activity
Biological Process
GO:0016310 phosphorylation
GO:0046677 response to antibiotic

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:3w0q, PDBe:3w0q, PDBj:3w0q
PDBsum3w0q
PubMed23747390
UniProtP00557|KHYB_ECOLX Hygromycin-B 4-O-kinase (Gene Name=hph)

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