Structure of PDB 3w0p Chain A

Receptor sequence
>3w0pA (length=295) Species: 562 (Escherichia coli) [Search protein sequence]
TSVEKFLIEKFGSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFY
KDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETEL
PAVLQPVAEVMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPH
VYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHAAFGSNNVLTDN
GRITAVIDWSEAMFGDPLYEVANIFFWRPWLACMEQQARYFERRHPELAG
SPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGT
3D structure
PDB3w0p Crystal structures of the ternary complex of APH(4)-Ia/Hph with hygromycin B and an ATP analog using a thermostable mutant.
ChainA
Resolution2.0 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.1.163: hygromycin B 4-O-kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP A L28 S29 V47 P75 R94 A95 I215 L20 S21 V39 P67 R86 A87 I207
BS02 HY0 A Q101 A198 S201 N231 W235 W238 L239 Q273 D285 Q93 A190 S193 N223 W227 W230 L231 Q265 D277
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0016301 kinase activity
Biological Process
GO:0016310 phosphorylation
GO:0046677 response to antibiotic

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:3w0p, PDBe:3w0p, PDBj:3w0p
PDBsum3w0p
PubMed23747390
UniProtP00557|KHYB_ECOLX Hygromycin-B 4-O-kinase (Gene Name=hph)

[Back to BioLiP]