Structure of PDB 3vti Chain A

Receptor sequence

>3vtiA (length=744) Species: 273068 (Caldanaerobacter subterraneus subsp. tengcongensis MB4)

QIQARQINIFGIVQGVGFRPFVFNIAQKYNLKGIVYNNSSGLYIEVEGEE
KDIEAFIREIKENPPSLSVIDEIQVREVEVKEYKDFKIVGSKEDGGFVPV
SPDMGVCEDCLRELKDPKDRRYRYPFINCTNCGPRFSIIEDIPYDRAKTS
MKVFPMCEKCSREYHDPHDRRFHAQPVACFDCGPSLSFVGEGCFDDEIKC
VAKALKEGKIVAIKGIGGFHLAVNALDDEAVATLRRRKKRYGKPFAVMMR
DVEEVKKYCIVSPEEERLLLSQRRPIVLLKKKGEKLAKGIADDLDTLGVM
LPYAPIHYLLMEEIDFPIVMTSGNVSEEPICKDNEEALEKLKDIADVFLL
NNRDIVNRIDDSVTSFNAGAERIIRRARGYAPQPILLKKEVKASILAVGG
FYKNTFCMTKGHYAFISHHIGDLDNEKAFNYYIEQIERYKKLFRVDPEVV
AHDMHKGYLSTQYAKSLDLPKIEVQHHHAHIASCMAEHNLDEKVIGIAYD
GTGYGTDGNVWGAEILVCDLKSFERIAHLKYKPLPGNELAIKKIYRTALG
FIFDNISFYKNFVEQVDSRELDIILKQIDRKINTAYVSSMGRFFDAVAAL
IGVRKEVLFEGQAAMELESLMAESEEYYEYEILKEDRYVIDPELILRQIY
EDYMKGFEKSYISAKFHNTVVNFTYDLANLIRKETGINKVVLSGGSFQNR
YLLRRLIEKLSLSGFEVYSNSKVPCNDGGISLGQAVIANKILEG

3D structure

• PDB: 3vti Structural basis for the reaction mechanism of S-carbamoylation of HypE by HypF in the maturation of [NiFe]-hydrogenases
• Chain: A
• Resolution: 2.56 Å

Enzymatic activity

• Enzyme Commision number: 3.6.1.7: acylphosphatase.
  6.2.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C114 C117 C136 C139	C107 C110 C129 C132
BS02	ZN	A	C164 C167 C186 C189	C157 C160 C179 C182
BS03	FE	A	H483 H487 D507 D734	H476 H480 D500 D727

Gene Ontology

Molecular Function

• GO:0003725 double-stranded RNA binding
• GO:0003998 acylphosphatase activity
• GO:0008270 zinc ion binding
• GO:0016743 carboxyl- or carbamoyltransferase activity
• GO:0016787 hydrolase activity
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0046944 protein carbamoylation
• GO:0051604 protein maturation

External links

• PDB: RCSB:3vti, PDBe:3vti, PDBj:3vti
• PDBsum: 3vti
• PubMed: 22740694
• UniProt: Q8RDB0