Structure of PDB 3vmf Chain A

Receptor sequence

>3vmfA (length=427) Species: 272557 (Aeropyrum pernix K1)

KPHMNLVVIGHVDHGKSTLVGHLLYRLGYIEEKKLKELEEQAKSRGKESF
KFAWILDKMKEERERGITIDLTFMKFETKKYVFTIIDAPGHRDFVKNMIT
GASQADAAILVVSARKGEFEAGMSTEGQTREHLLLARTMGIEQIIVAVNK
MDAPDVNYDQKRYEFVVSVLKKFMKGLGYQVDKIPFIPVSAWKGDNLIER
SPNMPWYNGPTLVEALDQLQPPAKPVDKPLRIPVQNVYSIPGAGTVPVGR
VETGVLRVGDKVVFMPPGVVGEVRSIEMHYQQLQQAEPGDNIGFAVRGVS
KSDIKRGDVAGHLDKPPTVAEEFEARIFVIWHPSAITVGYTPVIHVHTAS
VSSRIIEIKAKLDPKTGQVVEQNPQFLKAGDAAIVRFKPVKPLVVEKFSE
IPQLGRFAMRDMNRTVGIGIVTDVKPA

3D structure

• PDB: 3vmf Structural basis for translation termination by archaeal RF1 and GTP-bound EF1alpha complex
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D16 K19 S20 T71 H94
• Catalytic site (residue number reindexed from 1): D13 K16 S17 T68 H91

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GTP	A	V15 D16 G18 K19 S20 T21 S52 F53 I70 T71 G93 N152 K153 D155 S193 W195	V12 D13 G15 K16 S17 T18 S49 F50 I67 T68 G90 N149 K150 D152 S190 W192
BS02	MG	A	S20 T71	S17 T68

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3vmf, PDBe:3vmf, PDBj:3vmf
• PDBsum: 3vmf
• PubMed: 22772989
• UniProt: Q9YAV0|EF1A_AERPE Elongation factor 1-alpha (Gene Name=tuf)