Structure of PDB 3vhq Chain A

Receptor sequence

>3vhqA (length=385) Species: 69014 (Thermococcus kodakarensis KOD1)

NTIRVIVSVDKAKFNPHEVLGIGGHIVYQFKLIPAVVVDVPANAVGKLKK
MPGVEKVEFDHQAVLLGKPSWPAQTIPWGIERVKAPSVWSITDGSVSVIQ
VAVLDTGVDYDHPDLAANIAWCVSTLRGKVSTKLRDCADQNGHGTHVIGT
IAALNNDIGVVGVAPGVQIYSVRVLDARGSGSYSDIAIGIEQAILGPDGV
ADKDGDGIIAGDPDDDAAEVISMSLGGPADDSYLYDMIIQAYNAGIVIVA
ASGNEGAPSPSYPAAYPEVIAVGAIDSNDNIASFSNRQPEVSAPGVDILS
TYPDDSYETLMGTAMATPHVSGVVALIQAAYYQKYGKILPVGTFDDISKN
TVRGILHITADALGWDADYGYGVVRAALAVQAALG

3D structure

• PDB: 3vhq Requirement of Ca(2+) Ions for the Hyperthermostability of Tk-Subtilisin from Thermococcus kodakarensis
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D115 H153 N264 A324
• Catalytic site (residue number reindexed from 1): D105 H143 N254 A314
• Enzyme Commision number: 3.4.21.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	V108 Q110 A227 E229	V98 Q100 A217 E219
BS02	CA	A	L205 D208 V210 D226	L195 D198 V200 D216
BS03	CA	A	D212 D214 D216 I218 D222 D225	D202 D204 D206 I208 D212 D215
BS04	CA	A	Q84 D124 L164 N166 I168 V170	Q74 D114 L154 N156 I158 V160
BS05	CA	A	D214 D216 D222 D224	D204 D206 D212 D214

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3vhq, PDBe:3vhq, PDBj:3vhq
• PDBsum: 3vhq
• PubMed: 22686281
• UniProt: P58502|TKSU_THEKO Tk-subtilisin (Gene Name=TK1675)