Structure of PDB 3vgh Chain A

Receptor sequence
>3vghA (length=554) Species: 2287 (Saccharolobus solfataricus) [Search protein sequence]
YKIDGNEVIFTLWAPYQKSVKLKVLEKGLYEMERDEKGYFTITLNNVKVR
DRYKYVLDDASEIPDPASRYQPEGVHGPSQIIQESKEFNNETFLKKEDLI
IYEIHVGTFTPEGTFEGVIRKLDYLKDLGITAIEIMPIAQFPGKRDWGYD
GVYLYAVQNSYGGPEGFRKLVDEAHKKGLGVILDVVYNHVGPEGNYMVKL
GPYFSQKYKTPWGLTFNFDDAESDEVRKFILENVEYWIKEYNVDGFRLDA
VHAIIDTSPKHILEEIADVVHKYNRIVIAQSDLNDPRVVNPKEKCGYNID
AQWVDDFHHSIHAYLTGERQGYYTDFGNLDDIVKSYKDVFVYDGKYSNFR
RKTHGEPVGELDGCNFVVYIQNHDQVGNRGKGERIIKLVDRESYKIAAAL
YLLSPYIPMIFMGEEYGEENPFYFFSDFSDSKLIQGVREGRKKENGQDTD
PQDESTFNASKLSWKIDEEIFSFYKILIKMRKELSIACDRRVNVVNGENW
LIIKGREYFSLYVFSKSSIEVKYSGTLLLSSNNSFPQHIEEGKYEFDKGF
ALYK
3D structure
PDB3vgh Substrate recognition mechanism of a glycosyltrehalose trehalohydrolase from Sulfolobus solfataricus KM1.
ChainA
Resolution2.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L128 L131 G132 G154 D187 D252 Q283 H376 D377
Catalytic site (residue number reindexed from 1) L125 L128 G129 G151 D184 D249 Q280 H373 D374
Enzyme Commision number 3.2.1.141: 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A D153 N381 F427 D150 N378 F424
BS02 GLC A W150 Y152 W215 D252 Q283 H376 D377 W147 Y149 W212 D249 Q280 H373 D374
BS03 GLC A H255 Q283 D377 H252 Q280 D374
BS04 GLC A D308 H312 Y325 F352 D305 H309 Y322 F349
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0033942 4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0005992 trehalose biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3vgh, PDBe:3vgh, PDBj:3vgh
PDBsum3vgh
PubMed22334583
UniProtQ55088|TREZ_SACSO Malto-oligosyltrehalose trehalohydrolase (Gene Name=treZ)

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