Structure of PDB 3vgf Chain A

Receptor sequence
>3vgfA (length=555) Species: 2287 (Saccharolobus solfataricus) [Search protein sequence]
AYKIDGNEVIFTLWAPYQKSVKLKVLEKGLYEMERDEKGYFTITLNNVKV
RDRYKYVLDDASEIPDPASRYQPEGVHGPSQIIQESKEFNNETFLKKEDL
IIYEIHVGTFTPEGTFEGVIRKLDYLKDLGITAIEIMPIAQFPGKRDWGY
DGVYLYAVQNSYGGPEGFRKLVDEAHKKGLGVILDVVYNHVGPEGNYMVK
LGPYFSQKYKTPWGLTFNFDDAESDEVRKFILENVEYWIKEYNVDGFRLS
AVHAIIDTSPKHILEEIADVVHKYNRIVIAESDLNDPRVVNPKEKCGYNI
DAQWVDDFHHSIHAYLTGERQGYYTDFGNLDDIVKSYKDVFVYDGKYSNF
RRKTHGEPVGELDGCNFVVYIQNHDQVGNRGKGERIIKLVDRESYKIAAA
LYLLSPYIPMIFMGEEYGEENPFYFFSDFSDSKLIQGVREGRKKENGQDT
DPQDESTFNASKLSWKIDEEIFSFYKILIKMRKELSIACDRRVNVVNGEN
WLIIKGREYFSLYVFSKSSIEVKYSGTLLLSSNNSFPQHIEEGKYEFDKG
FALYK
3D structure
PDB3vgf Substrate recognition mechanism of a glycosyltrehalose trehalohydrolase from Sulfolobus solfataricus KM1.
ChainA
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L128 L131 G132 G154 D187 S252 E283 H376 D377
Catalytic site (residue number reindexed from 1) L126 L129 G130 G152 D185 S250 E281 H374 D375
Enzyme Commision number 3.2.1.141: 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A W150 Y152 W215 R250 S252 E283 H376 D377 W148 Y150 W213 R248 S250 E281 H374 D375
BS02 GLC A W150 D153 W215 N381 F427 W148 D151 W213 N379 F425
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0033942 4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0005992 trehalose biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3vgf, PDBe:3vgf, PDBj:3vgf
PDBsum3vgf
PubMed22334583
UniProtQ55088|TREZ_SACSO Malto-oligosyltrehalose trehalohydrolase (Gene Name=treZ)

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