Structure of PDB 3v8v Chain A

Receptor sequence
>3v8vA (length=664) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMW
SRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLND
TIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASI
ALDLSGDGLHLRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGS
GTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVKAEAQTRARKG
LAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPK
GPYGTVLSNPPYGDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLS
CLQLRADKQYKAKNGPLDCVQKNYHVAEMVAEDYTNRLRKNLKKFEKWAR
QEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPHKARQRLFDIIAATI
SVLGIAPNKLVLKTREKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIAR
RMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTTVDMSRTYLEWAERN
LRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFAFDVQRDHLAL
MKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFA
RNRQIHNCWLITAA
3D structure
PDB3v8v Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA
ChainA
Resolution2.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.173: 23S rRNA (guanine(2445)-N(2))-methyltransferase.
2.1.1.264: 23S rRNA (guanine(2069)-N(7))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A L218 R220 W223 L218 R220 W223
BS02 TRV A L218 H219 L218 H219
BS03 TRV A R582 G588 R552 G558
BS04 SAM A M197 C198 G199 S200 D262 S263 D290 V291 N309 M197 C198 G199 S200 D262 S263 D290 V291 N309
BS05 SAM A F546 Y548 D568 M569 D597 C598 F516 Y518 D538 M539 D567 C568
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0008990 rRNA (guanine-N2-)-methyltransferase activity
GO:0052915 23S rRNA (guanine(2445)-N(2))-methyltransferase activity
GO:0070043 rRNA (guanine-N7-)-methyltransferase activity
Biological Process
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0031167 rRNA methylation
GO:0032259 methylation
GO:0070475 rRNA base methylation
GO:0070476 rRNA (guanine-N7)-methylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3v8v, PDBe:3v8v, PDBj:3v8v
PDBsum3v8v
PubMed22362734
UniProtP75864|RLMKL_ECOLI Ribosomal RNA large subunit methyltransferase K/L (Gene Name=rlmL)

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