Structure of PDB 3uxm Chain A

Receptor sequence
>3uxmA (length=197) Species: 208964 (Pseudomonas aeruginosa PAO1) [Search protein sequence]
GLFVTLEGPEGTNRDYLAERLRERGIEVQLTREPGGTPLAERIRELLLAP
SDEPMAADTELLLMFAARAQHLAGVIRPALARGAVVLCDRFTDATYAYQG
GGRGLPEARIAALESFVQGDLRPDLTLVFDLPVEIGLARRLDRFEQEDRR
FFEAVRQTYLQRAAQAPERYQVLDAGLPLAEVQAGLDRLLPNLLERL
3D structure
PDB3uxm Structure guided development of novel thymidine mimetics targeting Pseudomonas aeruginosa thymidylate kinase: from hit to lead generation.
ChainA
Resolution1.95 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.4.9: dTMP kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A E158 D159 E147 D148
BS02 MG A E12 E156 E10 E145
BS03 0DN A E12 E39 P40 R50 L54 M70 R74 R96 A100 T101 Y104 Q105 F155 E10 E33 P34 R44 L48 M64 R68 R90 A94 T95 Y98 Q99 F144 MOAD: ic50=20uM
PDBbind-CN: -logKd/Ki=4.70,IC50=20uM
Gene Ontology
Molecular Function
GO:0004798 thymidylate kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
Biological Process
GO:0006227 dUDP biosynthetic process
GO:0006233 dTDP biosynthetic process
GO:0006235 dTTP biosynthetic process
GO:0009165 nucleotide biosynthetic process
GO:0016310 phosphorylation
GO:0046940 nucleoside monophosphate phosphorylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3uxm, PDBe:3uxm, PDBj:3uxm
PDBsum3uxm
PubMed22243413
UniProtQ9HZN8|KTHY_PSEAE Thymidylate kinase (Gene Name=tmk)

[Back to BioLiP]