Structure of PDB 3uxj Chain A

Receptor sequence
>3uxjA (length=261) Species: 243277 (Vibrio cholerae O1 biovar El Tor str. N16961) [Search protein sequence]
YANQYDPSLLQPVPRSLNRNDLHLSATLPFQGCDIWTLYELSWLNQKGLP
QVAIGEVSIPATSANLIESKSFKLYLNSYNQTRFASWDEVQTRLVHDLSA
CAGETVTVNVKSLNEYTAEPIVTMQGECIDDQDIEIANYEFDDALLQGAA
QGEEVSEVLHSHLLKSNCLITNQPDWGSVEIAYHGAKMNREALLRYLVSF
REHNEFHEQCVERIFTDIMRYCQPQSLTVYARYTRLGGLDINPFRSSHQS
APNHNQRMARQ
3D structure
PDB3uxj Crystal Structure of 7-cyano-7-deazaguanine reductase, QueF from Vibrio cholerae complexed with NADP and PreQ0
ChainA
Resolution1.401 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C194 T197 D201 H233 E234
Catalytic site (residue number reindexed from 1) C168 T171 D175 H207 E208
Enzyme Commision number 1.7.1.13: preQ1 synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PRF A E94 S95 C194 D201 F232 H233 E234 I267 E68 S69 C168 D175 F206 H207 E208 I241
BS02 NAP A S95 K96 K99 T197 L262 G263 S69 K70 K73 T171 L236 G237
Gene Ontology
Molecular Function
GO:0003674 molecular_function
GO:0016491 oxidoreductase activity
GO:0033739 preQ1 synthase activity
GO:0046857 oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
Biological Process
GO:0006400 tRNA modification
GO:0008150 biological_process
GO:0008616 queuosine biosynthetic process
Cellular Component
GO:0005575 cellular_component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3uxj, PDBe:3uxj, PDBj:3uxj
PDBsum3uxj
PubMed
UniProtQ9KTK0|QUEF_VIBCH NADPH-dependent 7-cyano-7-deazaguanine reductase (Gene Name=queF)

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