Structure of PDB 3uwk Chain A

Receptor sequence
>3uwkA (length=202) Species: 208964 (Pseudomonas aeruginosa PAO1) [Search protein sequence]
GLFVTLEGPEGAGKSTNRDYLAERLRERGIEVQLTREPGGTPLAERIREL
LLAPSDEPMAADTELLLMFAARAQHLAGVIRPALARGAVVLCDRFTDATY
AYQGGGRGLPEARIAALESFVQGDLRPDLTLVFDLPVEIGLARAAARDRF
EQEDRRFFEAVRQTYLQRAAQAPERYQVLDAGLPLAEVQAGLDRLLPNLL
ER
3D structure
PDB3uwk Structure guided development of novel thymidine mimetics targeting Pseudomonas aeruginosa thymidylate kinase: from hit to lead generation.
ChainA
Resolution1.91 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.4.9: dTMP kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 0DF A E12 E39 M70 R74 R96 A100 T101 Y104 Q105 F155 E10 E37 M68 R72 R94 A98 T99 Y102 Q103 F150 MOAD: ic50=58uM
PDBbind-CN: -logKd/Ki=4.24,IC50=58uM
Gene Ontology
Molecular Function
GO:0004798 thymidylate kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
Biological Process
GO:0006227 dUDP biosynthetic process
GO:0006233 dTDP biosynthetic process
GO:0006235 dTTP biosynthetic process
GO:0009165 nucleotide biosynthetic process
GO:0016310 phosphorylation
GO:0046940 nucleoside monophosphate phosphorylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3uwk, PDBe:3uwk, PDBj:3uwk
PDBsum3uwk
PubMed22243413
UniProtQ9HZN8|KTHY_PSEAE Thymidylate kinase (Gene Name=tmk)

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