Structure of PDB 3uus Chain A

Receptor sequence
>3uusA (length=732) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
LVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELRSHIQFYDGIKT
SDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPALYDH
VVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKY
LVQNRVTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKI
SLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIG
INAGRIRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLF
YPMWHLEVESLLVLKNNRGVEGNRVRHMDYGVQINKLMYTRLLKGEDITL
FSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQRVKAVELFSLMMQE
RASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLNDVNDE
NGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKR
GAMGRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKAS
NELAKEQGACPWFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRES
IKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGYVSIKASKDGILRQV
VPDYEHLHDAYELLWEMPGNDGYLQLVGIMQKFIDQSISANTNYDPSRFP
SGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDG
3D structure
PDB3uus Structural interconversions modulate activity of Escherichia coli ribonucleotide reductase.
ChainA
Resolution5.65 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C225 N437 C439 E441 C462 Y730 Y731
Catalytic site (residue number reindexed from 1) C220 N432 C434 E436 C457 Y725 Y726
Enzyme Commision number 1.17.4.1: ribonucleoside-diphosphate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 DTP A V7 K9 R16 I17 N18 I22 T55 I58 H59 F87 K91 V2 K4 R11 I12 N13 I17 T50 I53 H54 F82 K86
BS02 DTP A D232 S233 L234 I237 R262 I268 H275 T276 D227 S228 L229 I232 R257 I263 H270 T271
Gene Ontology
Molecular Function
GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016491 oxidoreductase activity
GO:0042802 identical protein binding
GO:0044183 protein folding chaperone
Biological Process
GO:0006457 protein folding
GO:0009185 ribonucleoside diphosphate metabolic process
GO:0009263 deoxyribonucleotide biosynthetic process
GO:0009265 2'-deoxyribonucleotide biosynthetic process
GO:0015949 nucleobase-containing small molecule interconversion
Cellular Component
GO:0005829 cytosol
GO:0005971 ribonucleoside-diphosphate reductase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3uus, PDBe:3uus, PDBj:3uus
PDBsum3uus
PubMed22160671
UniProtP00452|RIR1_ECOLI Ribonucleoside-diphosphate reductase 1 subunit alpha (Gene Name=nrdA)

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