Structure of PDB 3uq8 Chain A

Receptor sequence
>3uq8A (length=322) Species: 71421 (Haemophilus influenzae Rd KW20) [Search protein sequence]
NIQTQLDNLRKTLRQYEYEYHVLDNPSVPDSEYDRLFHQLKALELEHPEF
LTSDSPTQRVGAKPLSGFSQIRHEIPMLSLDNAFSDAEFNAFVARIEDRL
ILLPAPLTFCCEPKLDGLAVSILYVNGELTQAATRGDGTTGEDITANIRT
IRNVPLQLLTDNPPARLEVRGEVFMPHAGFERLNKYALEHNEKTFANPRN
AAAGSLRQLDPNITSKRPLVLNAYGIGIAEGVDLPTTHYARLQWLKSIGI
PVNPEIRLCNGADEVLGFYRDIQNKRSSLGYDIDGTVLKINDIALQNELG
FISKAPRWAIAYKFPAQEELTL
3D structure
PDB3uq8 Structure Guided Understanding of NAD(+) Recognition in Bacterial DNA Ligases.
ChainA
Resolution1.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E114 K116 D118 Y226 K315
Catalytic site (residue number reindexed from 1) E112 K114 D116 Y224 K313
Enzyme Commision number 6.5.1.2: DNA ligase (NAD(+)).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAD A Y18 Y22 D32 Y35 D36 F39 H40 K43 R154 Y16 Y20 D30 Y33 D34 F37 H38 K41 R152 MOAD: Kd=45uM
BS02 AMP A L82 K116 R137 E174 R201 Y226 V289 K291 L80 K114 R135 E172 R199 Y224 V287 K289
Gene Ontology
Molecular Function
GO:0003911 DNA ligase (NAD+) activity

View graph for
Molecular Function
External links
PDB RCSB:3uq8, PDBe:3uq8, PDBj:3uq8
PDBsum3uq8
PubMed22230472
UniProtP43813|DNLJ_HAEIN DNA ligase (Gene Name=ligA)

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