Structure of PDB 3uq2 Chain A

Receptor sequence

>3uq2A (length=318) Species: 9606 (Homo sapiens)

NLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACSI
PGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMW
YQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKA
AQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLR
QEGFLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYFT
GSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPGRVLPTPTEK
DVFRLLGLPYREPAERDW

3D structure

• PDB: 3uq2 The catalytic cycle for ribonucleotide incorporation by human DNA Pol lambda
• Chain: A
• Resolution: 2.25 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D175 D177 D233
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 Q372 V462 K463 G464 K472 R514 R517 K521 L527 S528 H530 H541	W22 Q120 V210 K211 G212 K215 R257 R260 K264 L270 S271 H273 H284
BS02	dna	A	W342 G343 A344 G345 K347 T348 D427 D429 D490 Y505 F506 G508 A510 N513	W90 G91 A92 G93 K95 T96 D175 D177 D233 Y248 F249 G251 A253 N256
BS03	dna	A	Y267 W274 R275 Y279 P303 G304 G306 R308 M309 K312	Y15 W22 R23 Y27 P51 G52 G54 R56 M57 K60
BS04	MG	A	D427 D429	D175 D177

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3uq2, PDBe:3uq2, PDBj:3uq2
• PDBsum: 3uq2
• PubMed: 22584622
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)