Structure of PDB 3ulk Chain A

Receptor sequence
>3ulkA (length=489) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVGCGAQGL
NQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQAD
LVINLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVV
MVAPKCPGTEVREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGG
HRAGVLESSFVAEVKSDLMGEQTILCGMLQAGSLLCFDKLVEEGTDPAYA
EKLIQFGWETITEALKQGGITLMMDRLSNPAKLRAYALSEQLKEIMAPLF
QKHMDDIISGEFSSGMMADWANDDKKLLTWREETGKTAFETAPQYEGKIG
EQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLHELPLIANT
IARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
GAVDNGQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAV
3D structure
PDB3ulk Bacterial and plant ketol-acid reductoisomerases have different mechanisms of induced fit during the catalytic cycle
ChainA
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K155 D217 E221
Catalytic site (residue number reindexed from 1) K155 D217 E221
Enzyme Commision number 1.1.1.86: ketol-acid reductoisomerase (NADP(+)).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NDP A G46 A47 Q48 R68 R76 S78 L105 T106 P107 D108 Q110 V114 G46 A47 Q48 R68 R76 S78 L105 T106 P107 D108 Q110 V114 MOAD: Kd=19.3uM
BS02 MG A D217 E389 E393 D217 E389 E393
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004455 ketol-acid reductoisomerase activity
GO:0008677 2-dehydropantoate 2-reductase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0042802 identical protein binding
GO:0046872 metal ion binding
GO:0050661 NADP binding
Biological Process
GO:0008652 amino acid biosynthetic process
GO:0009082 branched-chain amino acid biosynthetic process
GO:0009097 isoleucine biosynthetic process
GO:0009099 L-valine biosynthetic process
GO:0015940 pantothenate biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3ulk, PDBe:3ulk, PDBj:3ulk
PDBsum3ulk
PubMed23036858
UniProtP05793|ILVC_ECOLI Ketol-acid reductoisomerase (NADP(+)) (Gene Name=ilvC)

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