Structure of PDB 3uh1 Chain A

Receptor sequence

>3uh1A (length=367) Species: 559292 (Saccharomyces cerevisiae S288C)

AVTLHLRAETKPLEARAALTPTTVKKLIAKGFKIYVEDSPQSTFNINEYR
QAGAIIVPAGSWKTAPRDRIIIGLKEMPETDTFPLVHEHIQFAHCYKDQA
GWQNVLMRFIKGHGTLYDLEFLENDQGRRVAAFGFYAGFAGAALGVRDWA
FKQTHSDDEDLPAVSPYPNEKALVKDVTKDYKEALATGARKPTVLIIGAL
GRSGSGAIDLLHKVGIPDANILKWDIKETSRGGPFDEIPQADIFINCIYL
SKPIAPFTNMEKLNNPNRRLRTVVDVSADTTNPHNPIPIYTVATVFNKPT
VLVPTTAGPKLSVISIDHLPSLLPREASEFFSHDLLPSLELLPQRKTAPV
WVRAKKLFDRHCARVKR

3D structure

• PDB: 3uh1 Evidence in Support of Lysine 77 and Histidine 96 as Acid-Base Catalytic Residues in Saccharopine Dehydrogenase from Saccharomyces cerevisiae.
• Chain: A
• Resolution: 2.17 Å

Enzymatic activity

• Enzyme Commision number: 1.5.1.7: saccharopine dehydrogenase (NAD(+), L-lysine-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAI	A	A139 G200 G203 R204 S205 D227 T231 I250 Y251 V278 S279 D319 H320 L321	A137 G198 G201 R202 S203 D225 T229 I248 Y249 V276 S277 D317 H318 L319
BS02	SHR	A	R18 K77 F94 H96 K99 Q101 R131 A134 F135 S279 D281 D319 P322	R16 K75 F92 H94 K97 Q99 R129 A132 F133 S277 D279 D317 P320

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0004753 saccharopine dehydrogenase activity
• GO:0004754 saccharopine dehydrogenase (NAD+, L-lysine-forming) activity
• GO:0005515 protein binding
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0006553 lysine metabolic process
• GO:0009085 lysine biosynthetic process
• GO:0016558 protein import into peroxisome matrix
• GO:0019878 lysine biosynthetic process via aminoadipic acid

Cellular Component

• GO:0005737 cytoplasm
• GO:0005777 peroxisome

External links

• PDB: RCSB:3uh1, PDBe:3uh1, PDBj:3uh1
• PDBsum: 3uh1
• PubMed: 22243403
• UniProt: P38998|LYS1_YEAST Saccharopine dehydrogenase [NAD(+), L-lysine-forming] (Gene Name=LYS1)