Structure of PDB 3tup Chain A

Receptor sequence
>3tupA (length=404) Species: 9606 (Homo sapiens) [Search protein sequence]
GSVVELLGKSYPQDDHSNLTRKVLTRVGRNLHNQQHHPLWLIKERVKEHF
YKQYVGRFGTPLFSVYDNLSPVVTTWQNFDSLLIPADHPSRKKGDNYYLN
RTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAV
RLFSKHELFAGIKDGESLQLFEQSSRSAHKQETHTMEAVKLVEFDLKQTL
TRLMAHLFGDELEIRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQ
LVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSN
INQKVKFQPLSKYPAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVD
LIDKFVHPKTHKTSHCYRITYRHMERTLSQREVRHIHQALQEAAVQLLGV
EGRF
3D structure
PDB3tup Crystal Structure of Human Mitochondrial PheRS Complexed with tRNA(Phe) in the Active "Open" State.
ChainA
Resolution3.05 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G105 H119 R143 Q157 T235 A275
Catalytic site (residue number reindexed from 1) G94 H108 R132 Q146 T224 A264
Enzyme Commision number 6.1.1.20: phenylalanine--tRNA ligase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 rna A K33 D145 Q146 R294 K323 P325 A326 V327 I328 N329 D330 S332 D364 F366 C377 Q391 R395 R414 K22 D134 Q135 R283 K312 P314 A315 V316 I317 N318 D319 S321 D353 F355 C366 Q380 R384 R403
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004826 phenylalanine-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006432 phenylalanyl-tRNA aminoacylation
GO:0008033 tRNA processing
GO:0043039 tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

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Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:3tup, PDBe:3tup, PDBj:3tup
PDBsum3tup
PubMed22137894
UniProtO95363|SYFM_HUMAN Phenylalanine--tRNA ligase, mitochondrial (Gene Name=FARS2)

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