Structure of PDB 3tsu Chain A

Receptor sequence

>3tsuA (length=646) Species: 83334 (Escherichia coli O157:H7)

QIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPF
TVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQE
AALQAAIAQLKMGNIVAIKGIGGFHLACDARNSNAVATLRARKHRPAKPL
AVMLPVADGLPDAARQLLTTPAAPIVLVDKKYVPELCDDIAPGLNEVGVM
LPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALEDLQGIADGFLI
HNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGAD
LKNTFCLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALT
LDGIGMGENGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNL
LAQCLRFVPEWQNYPETASVAAANWSVLARAIERGINAPLASSCGRLFDA
VAAALGCAPATLSYEGEAACALEALAASCDGVTHPVTMPRVDNQLDLATF
WQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIH
NRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARWLAGE

3D structure

• PDB: 3tsu Structure of Hydrogenase Maturation Protein HypF with Reaction Intermediates Shows Two Active Sites.
• Chain: A
• Resolution: 1.92 Å

Enzymatic activity

• Enzyme Commision number: 3.6.1.7: acylphosphatase.
  6.2.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C112 C131	C12 C31
BS02	ZN	A	C181 C184	C81 C84
BS03	ZN	A	H475 H479 D502 D727	H375 H379 D402 D627
BS04	ADP	A	K402 H475 H479 D502 G503 I504 F598 A619 E623 G697 V698 N701 G726 D727	K302 H375 H379 D402 G403 I404 F498 A519 E523 G597 V598 N601 G626 D627
BS05	ANP	A	K243 R245 K248 P249 L277 E296 S322 N324 R372	K143 R145 K148 P149 L177 E196 S222 N224 R272

Gene Ontology

Molecular Function

• GO:0003725 double-stranded RNA binding
• GO:0008270 zinc ion binding
• GO:0016743 carboxyl- or carbamoyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0046944 protein carbamoylation

External links

• PDB: RCSB:3tsu, PDBe:3tsu, PDBj:3tsu
• PDBsum: 3tsu
• PubMed: 22153500
• UniProt: A0A0H3JHT3