Structure of PDB 3tm5 Chain A

Receptor sequence
>3tm5A (length=368) Species: 186497 (Pyrococcus furiosus DSM 3638) [Search protein sequence]
MKFLLTTAQGIEDIAKREVSLLLKKLGISFQIEEKPLGIEGRLLLEAEKA
YYVDEKGRKRELSISTYLNENSRLLHRVIIEIASEKFNGIEKDESEEALK
RIKDFVSSLPVEQFVKVSETFAVRSFRKGDHNITSIDIARTVGEAIFERL
SRFGTPLVNLDHPAVIFRAELIKDVFFLGIDTTGDSSLHKRPWRVYDHPA
HLKASIANAMIELAELDGGSVLDPMCGSGTILIELALRRYSGEIIGIEKY
RKHLIGAEMNALAAGVLDKIKFIQGDATQLSQYVDSVDFAISNLPYGLKI
GKSMIPDLYMKFFNELAKVLEKRGVFITTEKKAIEEAIAENGFEIIHHRV
IGHGGLMVHLYVVKLEHH
3D structure
PDB3tm5 Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.
ChainA
Resolution2.27 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.256: tRNA (guanine(6)-N(2))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SFG A H198 A200 H201 L202 M225 S228 T230 E248 K249 H253 D276 A277 N293 H198 A200 H201 L202 M225 S228 T230 E248 K249 H253 D276 A277 N293
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0008168 methyltransferase activity
GO:0016423 tRNA (guanine) methyltransferase activity
GO:0016740 transferase activity
GO:0160117 tRNA (guanine(6)-N2)-methyltransferase activity
Biological Process
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Cellular Component
External links
PDB RCSB:3tm5, PDBe:3tm5, PDBj:3tm5
PDBsum3tm5
PubMed22362751
UniProtQ8U248|TRM14_PYRFU tRNA (guanine(6)-N2)-methyltransferase (Gene Name=trm14)

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