Structure of PDB 3tlm Chain A

Receptor sequence

>3tlmA (length=987) Species: 9913 (Bos taurus)

MEAAHSKTTEECLAYFGVSETTGLTPDQVKRHLEKYGHNELPAEEGKSLW
ELVLEQFEDLLVRILLLAACISFVLAWFEEGEETVTAFVEPFVILLILIA
NAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDI
VEVAVGDKVPADIRILTIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAV
NQDKKNMLFSGTNIAAGKAIGIVATTGVGTEIGKIRDQMAATEQDKTPLQ
QKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWIRGAIYYFKIAV
ALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICS
DKTGTLTTNQMSVCKMFIIDRIDGDLCLLNEFSVTGSTYAPEGEVLKNDK
PVRSGQYDGLVELATICALCNDSSLDFNETKGIYEKVGEATETALTTLVE
KMNVFNTEVRNLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSP
AKSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPMTGPVKEKILSVIKE
WGTGRDTLRCLALATRDTPPKREEMVLDDSTKFMEYETDLTFVGVVGMLD
PPRKEVMGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEDVADR
AYTGREFDDLPLAEQREACRRACCFARVEPTHKSKIVEYLQSFDEITAMT
GDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGR
AIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDG
LPATALGFNPPDLDIMDRPPRTPKEPLISGWLFFRYMAIGGYVGAATVGA
AAWWFLYAEDGPHVTYSQLTHFMKCDFEGVDCEVFEAPQPMTMALSVLVT
IEMCNALNSLSENQSLVRMPPWVNIWLVGSIGLSMSLHFLILYVDPLPMI
FKLQALDLYHWLMVLKISLPVIGLDEILKFVARNYLE

3D structure

• PDB: 3tlm Crystal structure of sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) from bovine muscle.
• Chain: A
• Resolution: 2.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D351 D702 D706
• Catalytic site (residue number reindexed from 1): D351 D702 D706
• Enzyme Commision number: 7.2.2.10: P-type Ca(2+) transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	V304 A305 A306 I307 E309 N795 D799	V304 A305 A306 I307 E309 N795 D799
BS02	CA	A	N767 E770 T798 D799 E907	N767 E770 T798 D799 E902
BS03	MG	A	D351 T353 D702	D351 T353 D702
BS04	ACP	A	D351 T353 E442 F487 R489 M494 K514 A516 R559 L561 T624 G625 D626 R677	D351 T353 E442 F487 R489 M494 K514 A516 R559 L561 T624 G625 D626 R677

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005215 transporter activity
• GO:0005388 P-type calcium transporter activity
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006816 calcium ion transport
• GO:0006874 intracellular calcium ion homeostasis
• GO:0070588 calcium ion transmembrane transport
• GO:0106134 positive regulation of cardiac muscle cell contraction
• GO:1901896 positive regulation of ATPase-coupled calcium transmembrane transporter activity
• GO:1902082 positive regulation of calcium ion import into sarcoplasmic reticulum
• GO:1990036 calcium ion import into sarcoplasmic reticulum

Cellular Component

• GO:0016020 membrane
• GO:0016529 sarcoplasmic reticulum
• GO:0033017 sarcoplasmic reticulum membrane

External links

• PDB: RCSB:3tlm, PDBe:3tlm, PDBj:3tlm
• PDBsum: 3tlm
• PubMed: 22387132
• UniProt: Q0VCY0|AT2A1_BOVIN Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (Gene Name=ATP2A1)