Structure of PDB 3tfu Chain A

Receptor sequence
>3tfuA (length=417) Species: 1773 (Mycobacterium tuberculosis) [Search protein sequence]
GLTPEQIIAVDGAHLWHPYSSIGREAVSPVVAVAAHGAWLTLIRDGQPIE
VLDAMSSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKL
LVDITPAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGG
YHGDTFLAMSICDPHLAAQVFAPQVPRDYDPAYSAAFEAQLAQHAGELAA
VVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIATGFGRTGAL
FAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISAGAAGALMHG
PTFMANPLACAVSVASVELLLGQDWRTRITELAAGLTAGLDTARALPAVT
DVRVCGAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVYAMPPYICTP
AEITQITSAMVEVARLV
3D structure
PDB3tfu Mechanism-based Inactivation by Aromatization of the Transaminase BioA Involved in Biotin Biosynthesis in Mycobaterium tuberculosis.
ChainA
Resolution1.94 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) Y25 Y157 E220 D254 A257 K283 Y407
Catalytic site (residue number reindexed from 1) Y19 Y151 E204 D238 A241 K267 Y391
Enzyme Commision number 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PL8 A W64 G124 S125 Y157 H158 D254 I256 K283 W58 G118 S119 Y151 H152 D238 I240 K267 PDBbind-CN: -logKd/Ki=3.28,Ki=520uM
BS02 PL8 A P317 T318 P301 T302 PDBbind-CN: -logKd/Ki=3.28,Ki=520uM
Gene Ontology
Molecular Function
GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0008483 transaminase activity
GO:0030170 pyridoxal phosphate binding
Biological Process
GO:0009102 biotin biosynthetic process
Cellular Component
GO:0005737 cytoplasm

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3tfu, PDBe:3tfu, PDBj:3tfu
PDBsum3tfu
PubMed21988601
UniProtP9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)

[Back to BioLiP]