Structure of PDB 3t3n Chain A

Receptor sequence

>3t3nA (length=550) Species: 262724 (Thermus thermophilus HB27)

GPQDHVEIIPLGGMGEIGKNITVFRFRDEIFVLDGGLAFPEEGMPGVDLL
IPRVDYLIEHRHKIKAWVLTHGAEDHIGGLPFLLPMIFGKESPVPIYGAR
LTLGLLRGKLEEFGLRPGAFNLKEISPDDRIQVGRYFTLDLFRMTHSIPD
NSGVVIRTPIGTIVHTGDFKLDPTPIDGKVSHLAKVAQAGAEGVLLLIAD
ATNAERPGYTPSEMEIAKELDRVIGRAPGRVFVTTFASHIHRIQSVIWAA
EKYGRKVAMEGRSMLKFSRIALELGYLKVKDRLYTLEEVKDLPDHQVLIL
ATGSQGQPMSVLHRLAFEGHAKMAIKPGDTVILSSSPIPGNEEAVNRVIN
RLYALGAYVLYPPTYKVHASGHASQEELKLILNLTTPRFFLPWHGEVRHQ
MNFKWLAESMSRPPEKTLIGENGAVYRLTRETFEKVGEVPHGVLYVDGLG
VGDITEEILADRRHMAEEGLVVITALAGEDPVVEVVSRGFVKAGERLLGE
VRRMALEALKNGVREKKPLERIRDDIYYPVKKFLKKATGRDPMILPVVIE

3D structure

• PDB: 3t3n Molecular Basis for the Recognition and Cleavage of RNA by the Bifunctional 5'-3' Exo/Endoribonuclease RNase J.
• Chain: A
• Resolution: 3.09 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	I21 F43 D52 D79 F240 A241 R266 S267 T306 Q311 R318 I342 H372 S374 G375 H376	I17 F39 D48 D75 F236 A237 R262 S263 T302 Q307 R314 I338 H368 S370 G371 H372
BS02	ZN	A	D79 H80 D172 H398	D75 H76 D168 H394

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3t3n, PDBe:3t3n, PDBj:3t3n
• PDBsum: 3t3n
• PubMed: 21893286
• UniProt: Q72JJ7|RNJ_THET2 Ribonuclease J (Gene Name=rnj)