Structure of PDB 3t0f Chain A

Receptor sequence
>3t0fA (length=314) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
HHHGSMQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRYV
VDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDAT
CPLVTKVHMEVARASRRGEESILIGHAGHPDVEGTMGQYSNPEGGMYLVE
SPDDVWKLTVKNEEKLSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDD
ICYATTNRQEAVRALAEQAEVVLVVGSKNSSNSNRLAELAQRMGKRAFLI
DDAKDIQEEWVKEVKCVGVTAGASAPDILVQNVVARLQQLGGGEAIPLEG
REENIVFEVPKELR
3D structure
PDB3t0f Crystal Structures of Mutant IspH Proteins Reveal a Rotation of the Substrate's Hydroxymethyl Group during Catalysis.
ChainA
Resolution1.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.17.7.4: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 F3S A C12 G14 C96 L98 T167 C197 C17 G19 C101 L103 T172 C202
BS02 H6P A V40 H41 A73 H74 S225 S226 N227 S269 V45 H46 A78 H79 S230 S231 N232 S274
Gene Ontology
Molecular Function
GO:0016491 oxidoreductase activity
GO:0042380 hydroxymethylbutenyl pyrophosphate reductase activity
GO:0046872 metal ion binding
GO:0051538 3 iron, 4 sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding
GO:0051745 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Biological Process
GO:0008299 isoprenoid biosynthetic process
GO:0016114 terpenoid biosynthetic process
GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0050992 dimethylallyl diphosphate biosynthetic process
Cellular Component
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:3t0f, PDBe:3t0f, PDBj:3t0f
PDBsum3t0f
PubMed22137895
UniProtP62623|ISPH_ECOLI 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Gene Name=ispH)

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