Structure of PDB 3sx3 Chain A

Receptor sequence

>3sx3A (length=291) Species: 9606 (Homo sapiens)

FMVSLPRMVYPQPKVLTPCRKDVLVVTPWLAPIVWEGTFNIDILNEQFRL
QNTTIGLTVFAIKKYVAFLKLFLETAEKHFMVGHRVHYYVFTDQPAAVPR
VTLGTGRQLSVLEVRAYKRWQDVSMRRMEMISCERRFLSEVDYLVCVDVD
MEFRDHVGVEILTPLFGTLHPGFYGSSREAFTYERRPQSQAYIPKDEGDF
YYMGAFFGGSVQEVQRLTRACHQAMMVDQANGIEAVWHDESHLNKYLLRH
KPTKVLSPEYLWDQQLLGWPAVLRKLRFTAVPKNHQAVRNP

3D structure

• PDB: 3sx3 Sequence-dependent effects of cryoprotectants on the active sites of the human ABO(H) blood group A and B glycosyltransferases.
• Chain: A
• Resolution: 1.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H233 M266 W300 E303 A343 R352
• Catalytic site (residue number reindexed from 1): H170 M203 W237 E240 A280 R289
• Enzyme Commision number: 2.4.1.37: fucosylgalactoside 3-alpha-galactosyltransferase.
  2.4.1.40: glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UDP	A	F121 I123 Y126 V184 R188 D211 V212 D213 K346 H348 R352	F60 I62 Y65 V123 R127 D148 V149 D150 K283 H285 R289
BS02	GOL	A	S185 W300 H301 D302	S124 W237 H238 D239
BS03	GAL	A	H233 F236 T245 W300 E303	H170 F173 T182 W237 E240
BS04	MN	A	D211 D213	D148 D150

Gene Ontology

Molecular Function

• GO:0016758 hexosyltransferase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:3sx3, PDBe:3sx3, PDBj:3sx3
• PDBsum: 3sx3
• PubMed: 22349229
• UniProt: P16442|BGAT_HUMAN Histo-blood group ABO system transferase (Gene Name=ABO)