Structure of PDB 3sun Chain A

Receptor sequence

>3sunA (length=895) Species: 12353 (Escherichia phage RB69) [Search protein sequence]

MKEFYLTVEQIGDSIFERYIDSNGRERTREVEYKPSLFAHCPESQATKYF
DIYGKPCTRKLFANMRDASQWIKRMEDIGLEALGMDDFKLAYLSDTYNYE
IKYDHTKIRVANFDIEVTSPDGFPEPSQAKHPIDAITHYDSIDDRFYVFD
LLNSPYGNVEEWSIEIAAKLQEQGGDEVPSEIIDKIIYMPFDNEKELLME
YLNFWQQKTPVILTGWNVESFAIPYVYNRIKNIFGESTAKRLSPHRKTRV
KVIENMYGSREIITLFGISVLDYIDLYKKFSFTNQPSYSLDYISEFELNV
GKLKYDGPISKLRESNHQRYISYNIIAVYRVLQIDAKRQFINLSLDMGYY
AKIQIQSVFSPIKTWDAIIFNSLKEQNKVIPQGRSHPVQPYPGAFVKEPI
PNRYKYVMSFDLTSLYPSIIRQVNISPETIAGTFKVAPLHDYINAVAERP
SDVYSCSPNGMMYYKDRDGVVPTEITKVFNQRKEHKGYMLAAQRNGEIIK
EALHNPNLSVDEPLDVDYRFDFSDEIKEKIKKLSAKSLNEMLFRAQRTEV
AGMTAQINRKLLINSLAGALGNVWFRYYDLRNATAITTFGQMALQWIERK
VNEYLNEVCGTEGEAFVLYGDTDSIYVSADKIIDKVGESKFRDTNHWVDF
LDKFARERMEPAIDRGFREMCEYMNNKQHLMFMDREAIAGPPLGSKGIGG
FWTGKKRYALNVWDMEGTRYAEPKLKIMGLETQKSSTPKAVQKALKECIR
RMLQEGEESLQEYFKEFEKEFRQLNYISIASVSSANNIAKYDVGGFPGPK
CPFHIRGILTYNRAIKGNIDAPQVVEGEKVYVLPLREGNPFGDKCIAWPS
GTEITDLIKDDVLHWMDYTVLLEKTFIKPLEGFTSAAKLDYEKKA

3D structure

PDB

3sun Structure of the 2-Aminopurine-Cytosine Base Pair Formed in the Polymerase Active Site of the RB69 Y567A-DNA Polymerase.

Chain

Resolution

2.42 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	S360 I362 Y391 P392 G393 A394 N564 S565 G568 N572 W574 K705 R707 K800 K844 K874 K878	S360 I362 Y391 P392 G393 A394 N564 S565 G568 N572 W574 K705 R707 K800 K844 K874 K878	PDBbind-CN: Kd=9.2uM
BS02	dna	A	N284 D621 K706 Y708 M728 G729 K734 S735 S783 S784 N786 K790 Y791 K800 H804	N284 D621 K706 Y708 M728 G729 K734 S735 S783 S784 N786 K790 Y791 K800 H804	PDBbind-CN: Kd=9.2uM
BS03	TTP	A	D411 L412 S414 L415 Y416 R482 K560 T622 D623	D411 L412 S414 L415 Y416 R482 K560 T622 D623
BS04	CA	A	D411 L412 D623	D411 L412 D623
BS05	CA	A	D411 D623	D411 D623
BS06	CA	A	N505 N507 K531	N505 N507 K531

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0004527	exonuclease activity
GO:0008408	3'-5' exonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0039686	bidirectional double-stranded viral DNA replication
GO:0039693	viral DNA genome replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:3sun, PDBe:3sun, PDBj:3sun
PDBsum	3sun
PubMed	22023103
UniProt	Q38087\|DPOL_BPR69 DNA-directed DNA polymerase (Gene Name=43)

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