Structure of PDB 3si8 Chain A

Receptor sequence

>3si8A (length=422) Species: 9606 (Homo sapiens)

PHMATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGII
AVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVE
VMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIE
GLPQGVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIE
RETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKI
RSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGI
EHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKD
RNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKN
CNIQTEWSPPLTMLFLCATKFS

3D structure

• PDB: 3si8 Structure and mechanism of human DNA polymerase eta.
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W64 K86 K293 R313 P316 K317 T318 G320 C321 S322 N324 P326 G327 R351	Q40 Y41 W66 K88 K288 R308 P311 K312 T313 G315 C316 S317 N319 P321 G322 R346
BS02	dna	A	E116 K224 S257 G259 G260 K261 L262 R377 R382 R383 C384	E118 K219 S252 G254 G255 K256 L257 R372 R377 R378 C379
BS03	DZ4	A	D13 M14 C16 F18 I48 Y52 R55 R61 D115 K231	D15 M16 C18 F20 I50 Y54 R57 R63 D117 K226
BS04	3D1	A	S257 K293 N294 W297	S252 K288 N289 W292
BS05	MG	A	D13 M14 D115	D15 M16 D117
BS06	MG	A	D13 D115 E116	D15 D117 E118
BS07	CO	A	H393 H397	H388 H392

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:3si8, PDBe:3si8, PDBj:3si8
• PDBsum: 3si8
• PubMed: 20577208
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)