Structure of PDB 3sgl Chain A

Receptor sequence

>3sglA (length=630) Species: 632 (Yersinia pestis)

GLEETHHVFLKGNGFPARFASHPQQSCIFAETGFGTGLNFLTLWRDFALF
RQQSPNATLRRLHYISFEKYPLHVADLASAHARWPELASFAEQLRAQWPL
PLAGCHRILLADGAITLDLWFGDVNTLLPTLDDSLNNQVDAWFLDGFAPA
KNPDMWNEQLFNAMARMTRPGGTFSTFTAAGFVRRGLQQAGFNVTKVKGF
GQKREMLTGTLPQQIHAPTAPWYHRPAATRCDDIAIIGGGIVSALTALAL
QRRGAVVTLYCADAQPAQGASGNRQGALYPLLNGKNDALETFFTSAFTFA
RRQYDQLLEQGIAFDHQWCGVSQLAFDDKSRGKIEKMLHTQWPVEFAEAM
SREQLSELAGLDCAHDGIHYPAGGWLCPSDLTHALMMLAQQNGMTCHYQH
ELQRLKRIDSQWQLTFGQAAKHHATVILATGHRLPEWEQTHHLPLSAVRG
QVSHIPTTPVLSQLQQVLCYDGYLTPVNPANQHHCIGASYQRGDIATDFR
LTEQQENRERLLRCLPQVSWPQQVDVSDNQARCGVRCAIRDHLPMVGAVP
DYAATLAQYQDLSRRIDIAVAPVWPELFMVGGLGSRGLCSAPLVAEILAA
QMFGEPLPLDAKTLAALNPNRFWIRKLLKG

3D structure

• PDB: 3sgl Structural basis for hypermodification of the wobble uridine in tRNA by bifunctional enzyme MnmC.
• Chain: A
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 1.5.-.-
  2.1.1.61: tRNA 5-(aminomethyl)-2-thiouridylate-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	I270 G271 G272 G273 I274 V275 A295 D296 A303 S304 N306 A310 E434 L435 T465 G485 Y508 G569 L627 G628 S629 R630 G631 L632	I237 G238 G239 G240 I241 V242 A262 D263 A270 S271 N273 A277 E401 L402 T430 G450 Y473 G534 L583 G584 S585 R586 G587 L588
BS02	SAM	A	G66 F67 G68 T69 G70 L71 N72 E101 K102 D156 V157 D178 G179 F180	G33 F34 G35 T36 G37 L38 N39 E68 K69 D123 V124 D145 G146 F147

Gene Ontology

Molecular Function

• GO:0004808 tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
• GO:0008168 methyltransferase activity
• GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
• GO:0016491 oxidoreductase activity
• GO:0016645 oxidoreductase activity, acting on the CH-NH group of donors
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0002097 tRNA wobble base modification
• GO:0002098 tRNA wobble uridine modification
• GO:0008033 tRNA processing
• GO:0032259 methylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3sgl, PDBe:3sgl, PDBj:3sgl
• PDBsum: 3sgl
• PubMed: 23617613
• UniProt: Q8ZD36|MNMC_YERPE tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (Gene Name=mnmC)