Structure of PDB 3s61 Chain A

Receptor sequence

>3s61A (length=413) Species: 287 (Pseudomonas aeruginosa)

VVHDLIGVGFGPSNIALAIALQERAQAQGALEVLFLDKQGDYRWHGNTLV
SQSELQISFLKDLVSLRNPTSPYSFVNYLHKHDRLVDFINLGTFYPCRME
FNDYLRWVASHFQEQSRYGEEVLRIEPMLSAGQVEALRVISRNADGEELV
RTTRALVVSPGGTPRIPQVFRALKGDGRVFHHSQYLEHMAKPMKIAIIGG
GQSAAEAFIDLNDSYPSVQADMILRASALKPADDSPFVNEVFAPKFTDLI
YSREHAERERLLREYHNTNYSVVDTDLIERIYGVFYRQKVSGIPRHAFRC
MTTVERATATAQGIELALRDAGSGELSVETYDAVILATGYERQLHRQLLE
PLAEYLGDHEIGRDYRLQTDERCKVAIYAQGFSQASHGLSDTLLSVLPVR
AEEISGSLYQHLK

3D structure

• PDB: 3s61 Two Structures of an N-Hydroxylating Flavoprotein Monooxygenase: ORNITHINE HYDROXYLASE FROM PSEUDOMONAS AERUGINOSA.
• Chain: A
• Resolution: 3.03 Å

Enzymatic activity

• Enzyme Commision number: 1.14.13.195: L-ornithine N(5)-monooxygenase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	V16 G19 P20 S21 D45 K46 H53 Q64 I65 R106 V130 P168 G169 Y355 T407 L409	V8 G11 P12 S13 D37 K38 H45 Q56 I57 R98 V122 P160 G161 Y340 T392 L394
BS02	ORN	A	Q64 K69 N254 F257 S410	Q56 K61 N239 F242 S395
BS03	NDP	A	E62 Q64 R106 P175 G214 G215 G216 Q217 S218 E221 R240 N284 Y285 S286 A352 T353 G354	E54 Q56 R98 P167 G199 G200 G201 Q202 S203 E206 R225 N269 Y270 S271 A337 T338 G339

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0031172 ornithine N5-monooxygenase activity

Biological Process

• GO:0002049 pyoverdine biosynthetic process
• GO:0006879 intracellular iron ion homeostasis
• GO:0009058 biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:3s61, PDBe:3s61, PDBj:3s61
• PDBsum: 3s61
• PubMed: 21757711
• UniProt: Q51548|PVDA_PSEAE L-ornithine N(5)-monooxygenase (Gene Name=pvdA)