Structure of PDB 3s1s Chain A

Receptor sequence
>3s1sA (length=870) Species: 1408 (Bacillus pumilus) [Search protein sequence]
LNNCVFSYLPSYGDDEVSVYHPICEAALNQALVNTGLDSTYEVVHHELVG
SIEADFVIKNKQTKKYLLIVEVKRTKSQVSSTRYRLQAQSYVREANIKVE
QHYYCLTNLEIIDFFKHDPNKPVVSQQIIEPSPIVVGNFSDTVSEFYNRL
VEAFQNIIDISVNDAGTYKSSTANLVDILENRKDNSTSWHQALVVAGYEY
IRGVLRGQNVEVPTRDAIYFKSRPGRLLEEGRKIDFNVLFSEPEPNTNDN
DIWNVNLLSSLNDLGRRILTGDELAELIHDIATRGRGHEGVVPTDIELGK
VLSIISQHILGRPLTEDEVISDPAAGSGNLLATVSAGFNNVMPRQIWAND
IETLFLELLSIRLGLLFPQLVSSNNAPTITGEDVCSLNPEDFANVSVVVM
NPPYVSGVTDPAIKRKFAHKIIQLTGNRPQTLFGQIGVEALFLELVTELV
QDGTVISAIMPKQYLTAQGNESKAFREFLVGNFGLEHIFLYPREGLFEEV
IKDTVVFVGRKGSSVEEIEVLDSFTPLEQVDLHNLKRALSNSSNEQIIQP
MGMELRKEKREELENRVTVGWRHITSNGRVAEEWITNNLESHCIRLVASD
YDLRRGRVGNKGASDLLFINSKKKLWDLLDESVPRDWLYPALRKVNEINT
PIFNEDATPVRFLCPPNSAYQDGTGESIILDKILDVYVDFQVYKSKQKKF
EKSKEELKEILYKESDFYSSEHTVFIPRALRRSARAFINEQKVFCSTNAL
EVFGGNSEEMWLLLSWLSSVFAQLQFEAMAKDQEGERKLEKKSIQNLYIP
NLGDIDDVLKQDLIEEVREIHFFDLCRPRVRKLDLLWAKVFWSGNEMSKT
KEAAELLEDLVFERYPEGSQ
3D structure
PDB3s1s Characterization and crystal structure of the type IIG restriction endonuclease RM.BpuSI.
ChainA
Resolution2.35 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A E294 A329 A330 G331 S332 N334 D355 I356 V389 N406 P408 E289 A324 A325 G326 S327 N329 D350 I351 V384 N401 P403
BS02 MN A E21 D60 E76 V77 E16 D55 E71 V72
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0008168 methyltransferase activity
GO:0008170 N-methyltransferase activity
GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system
GO:0032259 methylation

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Molecular Function
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Biological Process
External links
PDB RCSB:3s1s, PDBe:3s1s, PDBj:3s1s
PDBsum3s1s
PubMed21724614
UniProtG1K3S1

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