Structure of PDB 3s1c Chain A

Receptor sequence

>3s1cA (length=498) Species: 4577 (Zea mays)

PWPASLAALALDGKLRTDSNATAAASTDFGNITSALPAAVLYPSSTADLV
ALLSAANSTPGWPYTIAFRGRGHSLMGQAFAPGGVVVNMASLGDAAAPPR
INVSADGRYVDAGGEQVWIDVLRASLARGVAPRSWTDYLYLTVGGTLSNA
GISGQAFRHGPQISNVLEMDVITGHGEMVTCSKQLNADLFDAVLGGLGQF
GVITRARIAVEPAPARARWVRLVYTDFAAFSADQERLTAPRPASFGPMSY
VEGSVFVNQSLATDLANTGFFTDADVARIVALAGERNATTVYSIEATLNY
DNATAAAAAVDQELASVLGTLSYVEGFAFQRDVAYAAFLDRVHGEEVALN
KLGLWRVPHPWLNMFVPRSRIADFDRGVFKGILQGTDIVGPLIVYPLNKS
MWDDGMSAATPSEDVFYAVSLLFSSVAPNDLARLQEQNRRILRFCDLAGI
QYKTYLARHTDRSDWVRHFGAAKWNRFVEMKNKYDPKRLLSPGQDIFN

3D structure

• PDB: 3s1c Kinetic and structural investigation of the cytokinin oxidase/dehydrogenase active site.
• Chain: A
• Resolution: 2.09 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H105 D169 E288
• Catalytic site (residue number reindexed from 1): H73 D137 E252
• Enzyme Commision number: 1.5.99.12: cytokinin dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	F61 F100 G102 R103 G104 H105 S106 Q110 A111 T168 D169 Y170 L173 T174 G176 G177 T178 S180 N181 G183 I184 V234 I235 W397 Y491 L492 S527	F29 F68 G70 R71 G72 H73 S74 Q78 A79 T136 D137 Y138 L141 T142 G144 G145 T146 S148 N149 G151 I152 V202 I203 W361 Y455 L456 S491
BS02	ZIR	A	D169 V378 E381 W397 L458	D137 V342 E345 W361 L422

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0019139 cytokinin dehydrogenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0009690 cytokinin metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3s1c, PDBe:3s1c, PDBj:3s1c
• PDBsum: 3s1c
• PubMed: 26519657
• UniProt: Q9T0N8|CKX1_MAIZE Cytokinin dehydrogenase 1 (Gene Name=CKX1)