Structure of PDB 3rv2 Chain A

Receptor sequence

>3rv2A (length=373) Species: 216594 (Mycobacterium marinum M)

GRLFTSESVTEGHPDKICDAVSDSVLDALLAADPRSRVAVETLVTTGQVH
VVGEVTTTAKEAFADITNIVRERILDIGYDSSDKGFDGASCGVNIGIGAQ
SPGDQGLMFGYAINDTPELMPLPIALAHRLSRRLTEVRKNGVLPYLRPDG
KTQVTIAYEDRVPVRLDTVVISTQHADDIDLVKTLDPDIREQVLKTVLDD
LAHDTLDASAVRVLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWARHGG
GAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERVEVQVAYAIGKAAPVG
LFVETFGSEAVDPVKIEKAIGEVFDLRPGAIIRDLNLLRPIYAPTAAYGH
FGRTDVDLPWERLDKVDDLKRAI

3D structure

• PDB: 3rv2 Increasing the structural coverage of tuberculosis drug targets.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H17 D19 K20 E45 E58 K181 F251 D259 A260 R265 K266 K286 K290 D292
• Catalytic site (residue number reindexed from 1): H13 D15 K16 E41 E54 K151 F221 D229 A230 R235 K236 K256 K260 D262
• Enzyme Commision number: 2.5.1.6: methionine adenosyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D394 D397 D398	D364 D367 D368

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0004478 methionine adenosyltransferase activity
• GO:0005524 ATP binding
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006556 S-adenosylmethionine biosynthetic process
• GO:0006730 one-carbon metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3rv2, PDBe:3rv2, PDBj:3rv2
• PDBsum: 3rv2
• PubMed: 25613812
• UniProt: B2HP50|METK_MYCMM S-adenosylmethionine synthase (Gene Name=metK)