Structure of PDB 3ruh Chain A

Receptor sequence

>3ruhA (length=336) Species: 703 (Plesiomonas shigelloides)

YMSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIGLDNFS
TGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAA
LGSVPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHP
ALPKVEENIGNPLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRR
QDPNGAYAAVIPKWTAAMLKGDDVYINGDGETSRDFCYIDNVIQMNILSA
LAKDSAKDNIYNVAVGDRTTLNELSGYIYDELNLIHHIKYREFRSGDVRH
SQADVTKAIDLLKYRPNIKIREGLRLSMPWYVRFLK

3D structure

• PDB: 3ruh Alternative analogs as viable substrates of UDP-hexose 4-epimerases
• Chain: A
• Resolution: 2.88 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S142 S143 S144 Y166 K170 N204
• Catalytic site (residue number reindexed from 1): S142 S143 S144 Y166 K170 N204
• Enzyme Commision number: 5.1.3.7: UDP-N-acetylglucosamine 4-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	G23 G26 F27 I28 D47 N48 S50 T51 G52 D78 I79 Q98 A99 A100 T117 A140 A141 S142 Y166 K170 Y193 V196	G23 G26 F27 I28 D47 N48 S50 T51 G52 D78 I79 Q98 A99 A100 T117 A140 A141 S142 Y166 K170 Y193 V196
BS02	UD6	A	G102 S103 S142 S143 S144 Y166 Y193 N195 A209 V210 K213 W214 Y225 I226 N227 R234 L271 R299 D302 V303 S306	G102 S103 S142 S143 S144 Y166 Y193 N195 A209 V210 K213 W214 Y225 I226 N227 R234 L271 R294 D297 V298 S301

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003974 UDP-N-acetylglucosamine 4-epimerase activity
• GO:0016853 isomerase activity

Biological Process

• GO:0009243 O antigen biosynthetic process

External links

• PDB: RCSB:3ruh, PDBe:3ruh, PDBj:3ruh
• PDBsum: 3ruh
• UniProt: Q7BJX9|GNE_PLESH UDP-N-acetylglucosamine 4-epimerase (Gene Name=wbgU)