Structure of PDB 3rj8 Chain A

Receptor sequence

>3rj8A (length=473) Species: 5520 (Microdochium nivale)

GAIEACLSAAGVPIDIPGTADYERDVEPFNIRLPYIPTAIAQTQTTAHIQ
SAVQCAKKLNLKVSAKSGGHSYASFGFGGENGHLMVQLDRMIDVISYNDK
TGIAHVEPGARLGHLATVLNDKYGRAISHGTCPGVGISGHFAHGGFGFSS
HMHGLAVDSVVGVTVVLADGRIVEASATENADLFWGIKGAGSNFGIVAVW
KLATFPAPKVLTRFGVTLNWKNKTSALKGIEAVEDYARWVAPREVNFRIG
DYGAGNPGIEGLYYGTPEQWRAAFQPLLDTLPAGYVVNPTTSLNWIESVL
SYSNFDHVDFITPQPVENFYAKSLTLKSIKGDAVKNFVDYYFDVSNKVKD
RFWFYQLDVHGGKNSQVTKVTNAETAYPHRDKLWLIQFYDRYDNNQTYPE
TSFKFLDGWVNSVTKALPKSDWGMYINYADPRMDRDYATKVYYGENLARL
QKLKAKFDPTDRFYYPQAVRPVK

3D structure

• PDB: 3rj8 Crystallization of carbohydrate oxidase from Microdochium nivale.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 1.1.3.-
  1.1.3.5: hexose oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	F29 A65 K66 S67 G68 G69 H70 S71 L88 G130 T131 C132 V135 G136 S138 G139 H140 F146 S192 I196 V197 Y425 N427 Y428	F29 A65 K66 S67 G68 G69 H70 S71 L88 G130 T131 C132 V135 G136 S138 G139 H140 F146 S192 I196 V197 Y425 N427 Y428
BS02	ZN	A	D306 H307	D306 H307
BS03	ZN	A	E231 D235	E231 D235
BS04	ZN	A	H114 D309	H114 D309

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0046562 glucose oxidase activity
• GO:0046872 metal ion binding
• GO:0047979 hexose oxidase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:3rj8, PDBe:3rj8, PDBj:3rj8
• PDBsum: 3rj8
• UniProt: I1SB12|MNCO_MICNN Carbohydrate oxidase (Gene Name=MnCO)