Structure of PDB 3req Chain A

Receptor sequence

>3reqA (length=725) Species: 1752 (Propionibacterium freudenreichii subsp. shermanii)

LPRFDSVDLGNAPVPADAARRFEELAAKAGTGEAWETAEQIPVGTLFNED
VYKDMDWLDTYAGIPPFVHGPYATMYAFRPWTIRQYAGFSTAKESNAFYR
RNLAAGQKGLSVAFDLPTHRGYDSDNPRVAGDVGMAGVAIDSIYDMRELF
AGIPLDQMSVSMTMNGAVLPILALYVVTAEEQGVKPEQLAGTIQNDILKE
FMVRNTYIYPPQPSMRIISEIFAYTSANMPKWNSISISGYHMQEAGATAD
IEMAYTLADGVDYIRAGESVGLNVDQFAPRLSFFWGIGMNFFMEVAKLRA
ARMLWAKLVHQFGPKNPKSMSLRTHSQTSGWSLTAQDVYNNVVRTCIEAM
AATQGHTQSLHTNSLDEAIALPTDFSARIARNTQLFLQQESGTTRVIDPW
SGSAYVEELTWDLARKAWGHIQEVEKVGGMAKAIEKGIPKMRIEEAAART
QARIDSGRQPLIGVNKYRLEHEPPLDVLKVDNSTVLAEQKAKLVKLRAER
DPEKVKAALDKITWAAGNPDDKDPDRNLLKLCIDAGRAMATVGEMSDALE
KVFGRYTAQIRTISGVYSKEVKNTPEVEEARELVEEFEQAEGRRPRILLA
KMGQDGHDRGQKVIATAYADLGFDVDVGPLFQTPEETARQAVEADVHVVG
VSSLAGGHLTLVPALRKELDKLGRPDILITVGGVIPEQDFDELRKDGAVE
IYTPGTVIPESAISLVKKLRASLDA

3D structure

• PDB: 3req Conformational changes on substrate binding to methylmalonyl CoA mutase and new insights into the free radical mechanism.
• Chain: A
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y89 Y243 H244 K604 D608 H610
• Catalytic site (residue number reindexed from 1): Y86 Y240 H241 K601 D605 H607
• Enzyme Commision number: 5.4.99.2: methylmalonyl-CoA mutase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	B12	A	L119 V206 R207 E247 G333 W334 A371 G609 H610 D611 R612 G613 I617 G653 S655 L657 G685 G686 Y705 T706 P707 T709	L116 V203 R204 E244 G330 W331 A368 G606 H607 D608 R609 G610 I614 G650 S652 L654 G682 G683 Y702 T703 P704 T706
BS02	ADN	A	Y89 Y243 E247 G333	Y86 Y240 E244 G330

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004494 methylmalonyl-CoA mutase activity
• GO:0005515 protein binding
• GO:0016853 isomerase activity
• GO:0016866 intramolecular transferase activity
• GO:0031419 cobalamin binding
• GO:0046872 metal ion binding

Biological Process

• GO:0019678 propionate metabolic process, methylmalonyl pathway

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3req, PDBe:3req, PDBj:3req
• PDBsum: 3req
• PubMed: 9655823
• UniProt: P11653|MUTB_PROFR Methylmalonyl-CoA mutase large subunit (Gene Name=mutB)