Structure of PDB 3rb0 Chain A

Receptor sequence

>3rb0A (length=341) Species: 273057 (Saccharolobus solfataricus P2)

GIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEA
RKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIE
IASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVF
AKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGIN
KLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIVT
MKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGR
TFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFI

3D structure

• PDB: 3rb0 Implications for damage recognition during Dpo4-mediated mutagenic bypass of m1G and m3C lesions.
• Chain: A
• Resolution: 3.225 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	K152 P184 G185 G187 N188 I189 T190 V296 S297 R298 R300 T301 K339	K152 P184 G185 G187 N188 I189 T190 V296 S297 R298 R300 T301 K339
BS02	dna	A	V32 G41 A42 V62 K78 G218 A220 R242 K243 S244 G246 R247 T250 K252 R253 K275 L293 R331 R332	V32 G41 A42 V62 K78 G218 A220 R242 K243 S244 G246 R247 T250 K252 R253 K275 L293 R331 R332
BS03	DGT	A	D7 Y10 F11 Y12 A44 T45 Y48 R51 D105 K159	D7 Y10 F11 Y12 A44 T45 Y48 R51 D105 K159
BS04	CA	A	D7 D105 E106	D7 D105 E106
BS05	CA	A	D7 F8 D105	D7 F8 D105
BS06	CA	A	A181 I186	A181 I186

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0042276 error-prone translesion synthesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3rb0, PDBe:3rb0, PDBj:3rb0
• PDBsum: 3rb0
• PubMed: 21645853
• UniProt: Q97W02|DPO4_SACS2 DNA polymerase IV (Gene Name=dbh)