Structure of PDB 3r0d Chain A

Receptor sequence

>3r0dA (length=422) Species: 83333 (Escherichia coli K-12)

ALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLV
IPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQR
AWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVA
FPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFAL
AQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSY
NGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLE
SGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLI
THHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKV
IASTQPAQTTVYLEQPEAIDYK

3D structure

• PDB: 3r0d Crystal structure of Cytosine Deaminase from Escherichia Coli complexed with two zinc atoms in the active site
• Chain: A
• Resolution: 1.501 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H61 H63 Q156 H214 E217 D313
• Catalytic site (residue number reindexed from 1): H58 H60 Q153 H211 E214 D310
• Enzyme Commision number: 3.5.4.-
  3.5.4.1: cytosine deaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PXN	A	W105 K109	W102 K106
BS02	ZN	A	H61 H63 H214	H58 H60 H211
BS03	ZN	A	H97 E138	H94 E135

Gene Ontology

Molecular Function

• GO:0004131 cytosine deaminase activity
• GO:0008198 ferrous iron binding
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
• GO:0035888 isoguanine deaminase activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006209 cytosine catabolic process
• GO:0019858 cytosine metabolic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:3r0d, PDBe:3r0d, PDBj:3r0d
• PDBsum: 3r0d
• UniProt: P25524|CODA_ECOLI Cytosine deaminase (Gene Name=codA)