Structure of PDB 3q2j Chain A

Receptor sequence
>3q2jA (length=261) Species: 1351 (Enterococcus faecalis) [Search protein sequence]
MRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGT
TYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYE
DEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLAD
VDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKV
SGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEK
IKYYILLDELF
3D structure
PDB3q2j Crystal structures of two aminoglycoside kinases bound with a eukaryotic protein kinase inhibitor.
ChainA
Resolution2.1501 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K44 D190 N195 D208
Catalytic site (residue number reindexed from 1) K41 D187 N192 D205
Enzyme Commision number 2.7.1.95: kanamycin kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CA A D153 D155 E157 E262 D150 D152 E154 E259
BS02 CKI A M26 Y42 M90 E92 A93 L97 S194 F197 I207 M23 Y39 M87 E89 A90 L94 S191 F194 I204 PDBbind-CN: -logKd/Ki=4.18,Ki=66.1uM
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008910 kanamycin kinase activity
GO:0016301 kinase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
Biological Process
GO:0016310 phosphorylation
GO:0046677 response to antibiotic

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Molecular Function
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Biological Process
External links
PDB RCSB:3q2j, PDBe:3q2j, PDBj:3q2j
PDBsum3q2j
PubMed21573013
UniProtP0A3Y5|KKA3_ENTFL Aminoglycoside 3'-phosphotransferase (Gene Name=aphA)

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