Structure of PDB 3pzc Chain A

Receptor sequence

>3pzcA (length=296) Species: 375 (Bradyrhizobium japonicum)

ADPLDHLADKLFHSMGSDGVYARTALYESIVERLAALITSHREAGTEALR
FPPVMSRAQLEKSGYLKSFPNLLGCVCGLHGTEREINAAVSRFDAGGDWT
TSLSPADLVLSPAACYPVYPIAASRGPLPKGGLRFDVAADCFRREPSKHL
DRLQSFRMREYVCIGTPDDVSDFRERWMVRAQAIARDLGLTFRVDYASDP
FFGRVGQMKAVSQKQQQLKFELLIPLRSEEQPTACMSFNYHREHFGTTWG
IQDANGEPAHTGCVAFGMDRLAVAMFHTHGTDLSAWPAKVRDILGL

3D structure

• PDB: 3pzc Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C131 R159 E161 R168 E176 K235 A250 S253 C279 R286
• Catalytic site (residue number reindexed from 1): C115 R143 E145 R152 E160 K219 A234 S237 C263 R270
• Enzyme Commision number: 6.2.1.n2: amino acid--[acyl-carrier-protein] ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C131 E176 C279	C115 E160 C263
BS02	GAP	A	R159 L169 F172 M174 E176 K235 A250 C251 M252 S253 G283 R286	R143 L153 F156 M158 E160 K219 A234 C235 M236 S237 G267 R270

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation

External links

• PDB: RCSB:3pzc, PDBe:3pzc, PDBj:3pzc
• PDBsum: 3pzc
• UniProt: Q89VT8|AACL1_BRADU Amino acid--[acyl-carrier-protein] ligase 1 (Gene Name=bll0957)