Structure of PDB 3px4 Chain A

Receptor sequence

>3px4A (length=549) Species: 1462 (Geobacillus kaustophilus)

KMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFLR
PETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLLA
AYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHL
VRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDT
KRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPVL
KKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVRP
ATKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESDW
LIFAADYSQIELRVLAHIAEDDNLMEAFRRDLYGIVYGISDYGLAQNLNI
SRKEAAEFIERYFESFPGVKRYMENIVQEAKQKGYVTTLLHRRRYLPDIT
SRNFNVRSFAERMAMNTPIQGSAADIIKKAMIDLNARLKEERLQAHLLLQ
VHDELILEAPKEEMERLCRLVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

3D structure

• PDB: 3px4 Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.
• Chain: A
• Resolution: 1.582 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T550 K551 T552 T556 A558 R578 Y587 R615 Q624 N625 I626 P627 I628 R629 V828 H829 D830	T253 K254 T255 T259 A261 R281 Y290 R318 Q327 N328 I329 P330 I331 R332 V501 H502 D503
BS02	dna	A	N527 N529 S530 K582 S585 T586 T611 Q612 T613 R615 S617 S618 T619 E620 N622 Y714 R771 F786 R789 M790 N793 Q797	N230 N232 S233 K285 S288 T289 T314 Q315 T316 R318 S320 S321 T322 E323 N325 Y387 R444 F459 R462 M463 N466 Q470
BS03	DCT	A	E469 Q470 D471 R472 L473 H768	E172 Q173 D174 R175 L176 H441

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:3px4, PDBe:3px4, PDBj:3px4
• PDBsum: 3px4
• PubMed: 22006298
• UniProt: Q5KWC1