Structure of PDB 3pwi Chain A

Receptor sequence
>3pwiA (length=440) Species: 444449 (Escherichia coli O157:H7 str. EC4042) [Search protein sequence]
TPVVTEMQVIPVAGHDSMLMNLSGAHAAFFTRNIVIIKDNSGHTGVGEIP
GGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQTFDL
RTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGN
RKATPLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKL
KGGVLAGEEEAESIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSL
AYAEDPCGAEQGFSGREVMAEFRRATGLPTATNMIATDWRQMGHTLSLQS
VDIPLADPHFWTMQGSVRVAQMCHEFGLTWGSHSNNHFDISLAMFTHVAA
AAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQVPEKPGLGVEIDMDQ
VMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR
3D structure
PDB3pwi Crystal structure of the mutant P34A of D-Glucarate dehydratase from Escherichia Coli complexed with product 5-keto-4-deoxy-D-Glucarate
ChainA
Resolution2.2296 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) K205 K207 D235 N237 E260 N289 M290 D313 H339 N341 I365
Catalytic site (residue number reindexed from 1) K199 K201 D229 N231 E254 N283 M284 D307 H333 N335 I359
Enzyme Commision number 4.2.1.40: glucarate dehydratase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D235 E260 N289 D229 E254 N283
BS02 GLR A N27 H32 T103 Y150 K207 D235 E260 N289 H339 N341 H368 R422 N21 H26 T97 Y144 K201 D229 E254 N283 H333 N335 H362 R416
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0008872 glucarate dehydratase activity
GO:0016829 lyase activity
GO:0046872 metal ion binding
Biological Process
GO:0019394 glucarate catabolic process
GO:0042838 D-glucarate catabolic process

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:3pwi, PDBe:3pwi, PDBj:3pwi
PDBsum3pwi
PubMed
UniProtP0AES2|GUDD_ECOLI Glucarate dehydratase (Gene Name=gudD)

[Back to BioLiP]