Structure of PDB 3puo Chain A

Receptor sequence

>3puoA (length=292) Species: 208964 (Pseudomonas aeruginosa PAO1)

MIAGSMVALVTPFDAQGRLDWDSLAKLVDFHLQDGTNAIVAVGTTGESAT
LDVEEHIQVVRRVVDQVKGRIPVIAGTGANSTREAVALTEAAKSGGADAC
LLVTPYYNKPTQEGMYQHFRHIAEAVAIPQILYNVPGRTSCDMLPETVER
LSKVPNIIGIKEATGDLQRAKEVIERVGKDFLVYSGDDATAVELMLLGGK
GNISVTANVAPRAMSDLCAAAMRGDAAAARAINDRLMPLHKALFIESNPI
PVKWALHEMGLIPEGIRLPLTWLSPHCHDPLRQAMRQTGVLA

3D structure

• PDB: 3puo Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa
• Chain: A
• Resolution: 2.65 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T44 Y107 Y133 R138 K161 I203
• Catalytic site (residue number reindexed from 1): T44 Y107 Y133 R138 K161 I203
• Enzyme Commision number: 4.3.3.7: 4-hydroxy-tetrahydrodipicolinate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	LYS	A	S48 A49 L51 D52 V53	S48 A49 L51 D52 V53
BS02	LYS	A	I266 L268 L270 T271	I266 L268 L270 T271

Gene Ontology

Molecular Function

• GO:0008840 4-hydroxy-tetrahydrodipicolinate synthase activity
• GO:0016829 lyase activity

Biological Process

• GO:0009085 lysine biosynthetic process
• GO:0009089 lysine biosynthetic process via diaminopimelate
• GO:0019877 diaminopimelate biosynthetic process
• GO:0044281 small molecule metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3puo, PDBe:3puo, PDBj:3puo
• PDBsum: 3puo
• PubMed: 21396954
• UniProt: Q9I4W3|DAPA_PSEAE 4-hydroxy-tetrahydrodipicolinate synthase (Gene Name=dapA)