Structure of PDB 3po4 Chain A

Receptor sequence

>3po4A (length=538) Species: 271 (Thermus aquaticus)

EEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRD
LKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRY
GGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHM
EATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVL
FDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKST
YIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRI
RRAFIAEEGWLLVALDYSQKELRVLAHLSGDENLIRVFQEGRDIHTETAS
WMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIE
RYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAA
ERKAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKE
RAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

3D structure

• PDB: 3po4 Learning from Directed Evolution: Thermus aquaticus DNA Polymerase Mutants with Translesion Synthesis Activity.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R487 T506 K508 T509 S513 T514 S515 A516 R536 K540 Y545 R573 Q582 N583 I584 P585 R587 H784	R193 T212 K214 T215 S219 T220 S221 A222 R242 K246 Y251 R279 Q288 N289 I290 P291 R293 H490
BS02	dna	A	N483 N485 S486 S543 T544 T569 A570 T571 S575 S576 S577 D578 N580 Y671 R728 R746 K747 N750 Q754	N189 N191 S192 S249 T250 T275 A276 T277 S281 S282 S283 D284 N286 Y377 R434 R452 K453 N456 Q460
BS03	DDS	A	R587 Q613 H639 R659 K663 F667 Y671	R293 Q319 H345 R365 K369 F373 Y377

Gene Ontology

Molecular Function

• GO:0001882 nucleoside binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair

External links

• PDB: RCSB:3po4, PDBe:3po4, PDBj:3po4
• PDBsum: 3po4
• PubMed: 21480455
• UniProt: P19821|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)