Structure of PDB 3pjg Chain A

Receptor sequence
>3pjgA (length=389) Species: 573 (Klebsiella pneumoniae) [Search protein sequence]
FMKITISGTGYVGLSNGVLIAQNHEVVALDIVQAKVDMLNQKISPIVDKE
IQEYLAEKPLNFRATTDKHDAYRNADYVIIATPTDYDPKTNYFNTSTVEA
VIRDVTEINPNAVMIIKSTIPVGFTRDIKERLGIDNVIFSPEFLREGRAL
YDNLHPSRIVIGERSARAERFADLLKEGAIKQDIPTLFTDSTEAEAIKLF
ANTYLALRVAYFNELDSYAESQGLNSKQIIEGVCLDPRIGNHYNNPSFGY
GGYCLPKDTKQLLANYESVPNNIIAAIVDANRTRKDFIADSILARKPKVV
GVYRLIMKSGSDNFRASSIQGIMKRIKAKGIPVIIYEPVMQEDEFFNSRV
VRDLNAFKQEADVIISNRMAEELADVADKVYTRDLFGND
3D structure
PDB3pjg Conformational change upon product binding to Klebsiella pneumoniae UDP-glucose dehydrogenase: a possible inhibition mechanism for the key enzyme in polymyxin resistance.
ChainA
Resolution2.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T118 E145 K197 N201 C253 D257
Catalytic site (residue number reindexed from 1) T119 E146 K198 N202 C254 D258
Enzyme Commision number 1.1.1.22: UDP-glucose 6-dehydrogenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 UGA A F142 L143 R144 K197 N201 V208 Y242 N244 S246 Y249 G250 Y252 M306 D388 F143 L144 R145 K198 N202 V209 Y243 N245 S247 Y250 G251 Y253 M307 D389 PDBbind-CN: -logKd/Ki=3.55,Ki=283uM
BS02 UGA A K256 D257 K284 Q319 G320 K323 K326 F345 K257 D258 K285 Q320 G321 K324 K327 F346 PDBbind-CN: -logKd/Ki=3.55,Ki=283uM
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0003979 UDP-glucose 6-dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0051287 NAD binding
Biological Process
GO:0000271 polysaccharide biosynthetic process
GO:0006065 UDP-glucuronate biosynthetic process

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Molecular Function
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Biological Process
External links
PDB RCSB:3pjg, PDBe:3pjg, PDBj:3pjg
PDBsum3pjg
PubMed21536136
UniProtA0A0J9WZA6

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